Fluorescence : One phenomenon, many applications Jussi Kinnunen, MSc (Physics), PhD student

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Fluorescence One phenomenon, many applications
Jussi Kinnunen, MSc (Physics), PhD student

• Oral presentation
• CREATE Gjøvik

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There will be

• Short introduction to fluorescence
• Few applications
• Something about bioanalytics
• Coloring or whitening merchandise
• Arts
• Measurements and data analysis
• by using two monochromator method

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What is fluorescence?
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For me it is fancy colours
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Light interaction with matter

• When light comes

• When light goes

• Absorption
• Time about 10-15 s
• Scattering
• Time about 0 s

• Fluorescence emission
• Lifetime about 10-8(10-10) s
• Lower energy photons than the one(s) absorbed
• Phosphorescence
• Lifetime gt10-8 s
• Lower energy photons than the one(s) absorbed
• Scattering
• Energy if the photon is close to the incident
  one(s)

A
F
P
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Applications Bioanalytics
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Fast detection of small quantities of anything

• Idea
• Choose a receptor for the target
• Attach label (reporter) to the receptor
• Illuminate and see
• Where is your target
• e.g. cancer cells
• Was there any of your target
• e.g. DNA sequencing

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Applications Industry
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Making things white
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Applications Art
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Masterpiece (Miera, 1950s), white light and
UVPhoto courtesy of Nick Padalino
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Color measurements of fluorescence
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Two monochromator method
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Measured data

• Donaldson matrix is calculated from the
  measured data
• The reflectance
• On diagonal
• The fluorescence
• Off diagonal

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Simulations
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Simulation with the scientific brown
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Simulation with the scientific brown
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Lights

• Simulations under
• Tungsten light (green)
• Daylight simulator (blue)
• White led (red)
• Fluorescent light (cyan)

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Under smooth light sources

• Halogen light

• Daylight simulator

The black curve is measured under polycromatic
light
fluorescence
total
reflectance
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Under peaky light sources

• Led light

• Fluorescent light

The black curve is measured under polycromatic
light
total
fluorescence
reflectance
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Thank you.

• www.uef.fi