(Animal Genetic Engineering)

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???
?????? (Animal Genetic Engineering)
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????
??? ??????? ??? ?????? ??? ??? ??? ????????? ???
??????
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??????????
??????,??????,???DNA????,???????,?????????????,???
????,??????????????????????????????????????,?????
????????????? ??????????DNA?????,??????????,?????
??,?????????
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?????????
????????4??? ???????????DNA??,??DNA?????????
?????????????DNA?????DNA?????DNA?????? ?????????
???????????
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??? ??????
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1 ???DNA??RNA???
??DNA???(?DNA????DNA???DNA) 1.
????????(??????)??????? ??????? 2.
????????????????K?? RNA?????????
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DNA????????-??????????--- RNA?????????????????
DEPC??
Genome DNA
Total RNA
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2 ???? ????(Gene library)??cDNA??(cDNA
library)??????(Genome library)?
?? cDNA???mRNA???,?????????DNA,??DNA???mRNA??,??
??DNA?cDNA? RT-PCR???PCR(Reverse Transcription
PCR)
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cDNA?? (cDNA library)
cDNA????????????mRNA,???????cDNA,????????????????
????????,?????????cDNA,??????,?????????mRNA???cDNA
????????????cDNA??? ????????????????
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????? (Genomic DNA Library) ????????????DNA
,?????????DNA??????????,???????????????,?????????,
?????????????????DNA??????????DNA??,????????,?????
???????????DNA????????,??????DNA????????????,?????
????DNA?????,?????????????,?????????????????DNA???
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????
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3 PCR
PCR,???????????????(Polymerase Chain
Reaction), ???????DNA????????????????
?1985????(K. Mullis)??,???????1993?????????
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PCR?????DNA??? ???????????????????
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PCR????? ?????dNTP????Mg2 Taq
DNA??? 1)??????? Thermusaquaticus2)??????3)Mg2?
??4)????? PCR?????? ????
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PCR???
???PCR ???PCR ???PCR
???PCR ????PCR ?????PCR ?????PCR
???PCR ???PCR (??)RT-PCR ???PCR
(??)RAPD - - - - - - - - - -
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????
??PCR???????????????????????,?????????? ??PCR???
??????????,???PCR???????PCR?????,??PCR????????????
??????????DNA ??PCR??????????????????????????
?DNA?
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????
??PCR????PCR???????PCR??????,???????,???????????,
???????????????????? ????PCR???????????mRNA3???
???????(polyA)??,?????oligo(dT)?????????????mRNA??
??cDNA?
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4 ???? ????(gene cloning)?????,?????D
NA??,???????,?????????DNA?????????????????????,???
?????????????,???????DNA?????? ??(clone)??????DNA
?????????,???DNA???????????????????(cloning)?
??????1) ??????DNA??(??)2)????????????3)??DNA?
???????4)??????????5)???????/????
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5 ?????? 1?PCR???? 2???????
(????????)
Southern?? Northern?? ????
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????
Sanger?(1977)?????????????
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Southern??
?southern?1975???????DNA?????????????,???Souther
n??(Southern Blot)? ???????DNA??DNA??,????????
?????
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Northern??
???Northern??(Northern Blot),?????????RNA(???mRN
A)?????????? ??DNA???(Southern
??)???,?????Northern???
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????
???????????,??????? ????????????????
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Western(??)??
???????????,???????,??????????????????,?????????
?,??????????????? ?DNA?RNA????Southern???Norther
n?????,??Western??? ???Northern????,?????????
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??? ????????
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1 ??????
???????DNA??????????????????? ???????????????????
???? DNA?
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1)???????00522)????pBudCE4.13)??Invitrogen4)
????????5) ????6)??????7)????PM
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?????????
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2 ???????

1. ????????????????,??????????????
2. PCR
3. ???

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3 ????
??????????????
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???????????????????????
??????????
???????pEGFP????
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??? ????? (Transgenic technology)
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1 ????????? ?????? 1982?,?????????????????
?????????????,????????????????????,??????????????
???????????????????????23?,??????
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?? ??? 1)??????????????????????,?????????,??????
?????????,????????? 2)?????????????????,??????????
??????,?????????,?????????????????????????????????
???? ????? ??????????????????????????????????????
??????????
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?????? 1???????DNA??????????,?????DNA?????????
?,??????????? 2????????????????????????????????
?????????????????????,???????
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??????
3.??????????????????????,?????????????RNA?????,??
??????????????????????????,??????????????,????????
?? 4.???????????????????????,?????????,?????????
??????????????,?????????????????,????????
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?????????? 1???????????,?????????????? 2????????
????,???????????? 3?????????,????????? 4??????????
???????????????????????????????????
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??????????? 2002???
??????????????????99? ???66,?3900???,??????????
???,?????75????34????71??????????? ????23,?135
0???? ????6,?350???? ?????,?4,?210???? ????????
?20???,0.3???
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???? 1??????? 1)????????? 2)??????????? 2?????
1)?? 2)??? 3)?????????????
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????????? ????????????????????????????????????
????????????????,???????????????????
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???????????
?????????????????????????????,????????(????)????
??????????????????????????????????????????,2010???
?????????????????500??????
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??? ????????? (Gene Knockout Gene Therapy)
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?????????? ????(gene knock out)?80????????????
??????????????,???????????????????????,???????????
?????,?????????
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??
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(No Transcript)
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????????? 1???????????? 2???????????????,????
????? 3????????,??????/?????,??????? 4??????,???
??? 5???????????????
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?????????
????(gene therapy) ???????????????????????????????
????????????????(?????????????????),??????????????
????? ???????DNA??,????????????????,??????????,???
???????????,???????????
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???????
???????????????????????????????,??????????????,???
????????????????? ?????????????????,??????????????
???????????(gene therapeutics)? ???????????????,??
?RNAi?????????
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??? ?????? (Animal Cloning Technology)
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Creating Dolly!
1997?2?22?,??Nature?????????????????????,?????
????,????????
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???????
1998?4?????????????,????????????2003.2.14?????????
??????????,???????????????,???????????????? ?
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??????????????????????,???????????????????,???????
??????,???????????????????277???,?????????1996?7?5
?,?????????1997?2?23???????
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?????? ????????????????
????????????????????????????????????????,??????,?
?????????????,????????????????????,???????????????
?????,???????????????????????? ???????????????,??
???????
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???????
1???????????????
?????
???
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???????
2????????????? (AI?Clone) 3?????????
4??????????????????