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Measuring gene expression

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Measuring gene expression RNA blotting RT-PCR RT-PCR RT-PCR 10 copies 100 1,000 10,000 100,000 1,000,000 10,000,000 28 cycles 4 x 103 copies Green fluorescent protein ... – PowerPoint PPT presentation

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Title: Measuring gene expression


1
Measuring gene expression
2
RNA blotting
3
RT-PCR
4
RT-PCR
5
RT-PCR
10 copies
100
1,000
10,000
100,000
1,000,000
10,000,000
6
Green fluorescent protein
7
Macroarray
human cDNAs spotted on membrane
thymus mRNA
8
Microarray
Arabidopsis cDNAs spotted on slide
root mRNA probe
amount of mRNA
9
RNA from control plants YELLOW
RNA from salt-treated plants
RED
10
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