???? RNA????? Chapter 11 Biosynthesis of RNA

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???? RNA????? Chapter 11 Biosynthesis of RNA

• RNA???????
• RNA???????
• RNA?????????
• ????RNA????????

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• ????
• ??RNA???????
• ??RNA???????
• ???????
• ??????????????
• ??????
• RNA???????
• ????????
• ????2

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• ?RNA???????,???DNA??????RNA,???DNA???????????RNA?
  ???????
• ??????RNA???,????rRNA,tRNA,mRNA,snRNA?HnRNA?

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??? RNA???????

• ?????????
• ??(transcription)???????????DNA?????????????,?????
  ???DNA???RNA?

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• ?????????,???????????????DNA???????RNA???DNA??????
  ?(???),?????????DNA???????(???)?

????
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????
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• ????????
• RNA?????,?DNA????,?RNA???????,??????RNA?,??RNA????
  ???????
• ???RNA?,????????????,??????????????????,????????

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• ????????
• RNA?????,???????????,??????DNA?????3'?5',?RNA?????
  ??5'?3'?

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• ?????????????
• RNA?????,???DNA???????????,??????????????????,????
  ??????????DNA?????????,?????????????????

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??? RNA???????

• ????
• ???????,?ATP,GTP,CTP,UTP?
•  
• ????
• ?????DNA?????

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• ??RNA???(DDRP)
• ?????????????DNA?RNA?????????DNA?????????????????,
  ?????,???5'?3'??RNA?

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• ??????RNA????????????,?a2ßß's?
• s?????????????,????????????,?????(a2ßß')??????,?RN
  A???????

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????RNA?????
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• ??????RNA???????a-????????????,????1012??????????
  ?,??????,???????

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??RNA??????
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• ??????
• 1.??????????????,???????50kd?????????????,???R
  NA????,??RNA????
• 2.nusA???????69kd?????,???RNA?RNA??????,?????
  ???????

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• ??????
• ???????????????????(CAP),???cAMP????(CRP)?????????
  ?,??????23kd?????cAMP???,??RNA??????????,?????????
  

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??? RNA?????????

• ????
• ????RNA?????s?????????,????????????????
• ???????????????-35??TTGACA???
• ???????,????-10??TATAAT??(Pribnow?) ,??????

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???????????????
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• ??????,?RNA??????????????????DNA???????(promoter)?
  

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• ???????????????????TA???,???Hogness??TATA??
• ????,??????????????????,?CAAT??GC???

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• ??????????????????RNA?????????????????????????????
  ???????????(trans-criptional factor, TF)???
  TF?A,TF?B,TF?D,TF?E,TF?F,TF?-I?

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????RNA????????????

• ???? ? ?
• TF?A ??TF?D??
• TF?B ??pol ???
• TF?D ??TATA?
• TF?E ATPase
  
• TF?F ???

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• ????
• RNA?????????????,???ATP?GTP????????????,?????3',5'
  -??????

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• ????
• s????????,?????????DNA???,????????,????RNA?

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• ????
• RNA????????????
• 1.??????DNA?????????????????GC???,?RNA???????
  ?U?????????,??RNA??????????,??RNA??????
• 2.??????????????(???)?????????,???RNA????

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(No Transcript)
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??? ????RNA????????

• ??mRNA??????
• 1.??(adding cap)
• ??mRNA?5'-???m7GTP??????????????,?HnRNA???????
• ???????????????,?5'-???????,??????????,??GpppN???,
  ??G??????

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(No Transcript)
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• 2.??(adding tail)
• ????????????,??????????3'-?????????,?????polyA?pol
  yA???mRNA???????

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• 3.??(splicing)
• ?????????????????????????????????????????????,????
  ????????????????????
• ????HnRNA??????snRNA???????????,??????????????????
  

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(No Transcript)
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• 4.?????
• ???????,?????????????

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????mRNA????????
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??tRNA??????

• ???????????
• ???
• ???
• ?????

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??rRNA??????
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????rRNA?????
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(No Transcript)