The SOP

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The SOP

• Technicians working in a biotechnology
  manufacturing facility must follow procedures
  exactly every time
• Companies, therefore, produce detailed
  step-by-step instructions called a Standard
  Operating Procedure, or SOP
• Is a very formal set of instructions, including
  precautions and lists of necessary materials.
• Well-written SOPs provide direction, improve
  communication, reduce training time and improve
  work consistency
• Forces a person to think through a procedure step
  by step - it becomes a useful training
  took and reminder of GLPs
• Every company has its own criteria for writing
  SOPs, and some are very detailed

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Topics That Lend Themselves to SOP Format

• Lab safety
• Disposal of hazardous materials
• Surface Decontamination
• Spill Procedure
• Inventory Procedures
• Operation and maintenance of equipment
• example how to run a PCR reaction
• using a spectrophotometer

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• Freestall MaintenanceEffective date
  10/1/2001Developed by Milking staff
• Key Objectives
• Clean stalls to create a sanitary bed for cows to
  lie on
• Distribute bedding evenly to promote cow comfort
• Notes
• Freestall rake is needed for this job
• Freestall maintenance should be completed
  immediately after a group of cows is moved to the
  holding area
• The Barn Operator is responsible for freestall
  maintenance
• Scrape any manure or wet bedding from the
  freestall beds into the alley.
• Rake bedding from front of the freestall beds
  toward the back.
• Evenly distribute the bedding so that at least 1
  inch of material covers all parts of the bed.
• Observe free stall beds, divider loops, and other
  parts of the barn for any foreign objects, loose
  bolts, or other unusual conditions.
• Return freestall rake to storage area at end of
  barn.
• Write any unusual observations or repairs needed
  in the milking shift notebook.

EXAMPLE - AGRICULTURAL
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EXAMPLE 2 - PCR Protocol
Standard Protocol 55ul Total PCR Reaction
Volume Using gloved hands, remove the master
mixes from the freezer. Allow the tubes to thaw
then gently vortex to mix. In a containment hood
or dead air box remove an appropriate aliquot to
clean, sterile microfuge tube (one tube for each
of the master mixes). Aliquot volumes should be
50ul for each sample 150ul for the positive,
negative and no template controls. We recommend
adding an additional 20ul to correct for
pipetting errors. Add the appropriate amount of
either AmpliTaq Gold or AmpliTaq DNA polymerase
(0.25ul of either AmpliTaq Gold or AmpliTaq _at_
5U/ul per 55ul total PCR reaction volume) to each
of the master mixes and gently mix by inverting
several times or gently vortexing. Aliquot 50ul
of master mix to individual wells of a PCR plate.
Add 5ul of DNA from the unknown and control
samples to individual tubes or wells containing
the respective master mix reactions, and pipette
up and down several times to mix. Amplify target
DNA using the universal thermocycler program.
Continued on next slide
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Specimen Requirements This assay tests genomic
DNA or complementary DNA (cDNA) from the
following sources 5cc of peripheral blood, bone
marrow biopsy, or bone marrow aspirate
anti-coagulated with heparin or EDTA Minimum 5mm
cube of tissue 2ug of genomic DNA 5ug of total
RNA or mRNA 1ug of cDNA Formalin-fixed paraffin
embedded tissue or slides
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SOP Writing Activity

• We will form 10 groups and each will be
  responsible for writing an SOP for a common
  activity.
• Each group will present there SOP to the class
  for constructive criticism.

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Groups

1. Ricky Emily
2. Alex ? Lindsey
3. Kristen Yuriy
4. Lauren Devin
5. Zach Liz
6. Amanda Kat
7. Avelina Ryan
8. Dylan Alex ?
9. Andrew Allie
10. Rawad Scott Megan

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List of SOPs

• Making a PBJ sandwich
• Making Mac and Cheese
• Taking a shower
• Changing a flat tire
• Coming to school in the morning
• Solve the following algebraic equation
• (3y 7)/5 (2y-12)/3
• Loading dishes in the dishwasher
• Walk from here to the front of the building
• Properly arrive at an airport and board a plane
• Build a fire and make smores