Fast Protein Liquid Chromatography (FPLC)

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Fast Protein Liquid Chromatography (FPLC)
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1. Packing

• Column components

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Gel degassing.
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Stopping the outlet of the column.
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Filling liquid in the bottom of the column
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Pouring gell slurry to the column.
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Settling of gels.
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Adjusting the adaptor
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Fixed adaptor
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Conneting a line to the adaptor
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Adaptor touching the upper layer of gels.
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Gel level lowers due to the solution flow into
the column.
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Stopping the outlet line
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Connetion of pump line to the adaptor
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Blue dextran solution flows in the column.
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2. Valve control
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Injection of sample
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3. Remote controller
FPLC controller.
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Pump front panel.
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Pump flow rate calibration
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Manual mode of gradient setting
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Valve number setting
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Flow rate setting
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4. Multimeter
Multimeter for digital data aquisition
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5. Detector
UV detector
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6. Software(PROTEK 506)
Software UV signal data aquisition
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Multimeter setting for sending data to notebook
computer
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Sampling time setup
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File name.
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Save data file In a folder
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Starting data acquisition End ing data collection