Enzyme Kinetics II - PowerPoint PPT Presentation

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Enzyme Kinetics II

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Title: Introduction to enzymes Author: William R. Widger Last modified by: Glen Legge Created Date: 9/12/1998 6:36:47 PM Document presentation format – PowerPoint PPT presentation

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Title: Enzyme Kinetics II


1
Enzyme Kinetics II
  • 10/16/2008

2
Kinetic data cannot unambiguously establish a
reaction mechanism.
Although a phenomenological description can be
obtained the nature of the reaction intermediates
remain indeterminate and other independent
measurements are needed.
3
Reaction MechanismsA Sequential Reactions
  • All substrates must combine with enzyme before
    reaction can occur

4
Bisubstrate reactions
5
Random Bisubstrate Reactions
6
Ping-Pong Reactions
  • Group transfer reactions
  • One or more products released before all
    substrates added

7
Inhibition kinetics
  • There are three types of inhibition kinetics
    competitive,
  • mixed and uncompetitive.
  • Competitive- Where the inhibitor competes with
    the substrate.

8
Competitive Inhibition
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HIV protease inhibitors
12
Competitive Inhibition Lineweaver-Burke Plot
13
Uncompetitive Inhibition
14
Uncompetitive Inhibition Lineweaver-Burke Plot
15
Mixed inhibition
16
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17
Mixed inhibition is when the inhibitor binds to
the enzyme at a location distinct from the
substrate binding site. The binding of the
inhibitor will either alter the KM or Vmax or
both.
18
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19
The effect of pH on kinetic parameters
20
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22
Lecture 17Tuesday 10/21/08Enzyme Kinetics /
Exam II Minireview
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