Spectrophotometry-III - PowerPoint PPT Presentation

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Spectrophotometry-III

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Title: Slide 1 Author: Alex Last modified by: NAZAREAY Created Date: 4/13/2005 3:07:25 AM Document presentation format: On-screen Show Company: BSC Other titles – PowerPoint PPT presentation

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Title: Spectrophotometry-III


1
Lecture 19
  • Spectrophotometry-III

2
Monochromator (filter, wavelength selector)
Light Source
Detector
Sample
Spectrometer
Data Processing
3
Single beam
Double beam
4
A tungsten lamp
3000 K
5
UV sources
Deuterium Lamp
Deuterium higher intensity
6
Limits to Beers Law
Incident light
Emergent light
P1
P0
Stray light
7
(No Transcript)
8
Solvents for UV/vis Spectrophotometry
Solvent Lower Wavelength
Limit Water 180 nm Ethanol 220
nm Hexane and 200 nm Cyclohexane Acetone 320
nm
9
(No Transcript)
10
Bugert, Lambert and Beer
Beers law
A
Straight line!
concentration
11
A or ?
Measure _at_ this wavelength!
Other components of the mixture
B
Wavelength, nm
12
Limits to Beers Law
Polychromatic light (several wavelength)
13
A or ?
Other components of the mixture
Intensity is too low!
B
Wavelength, nm
14
A or ?
Other components of the mixture
B
Measurement is difficult
Wavelength, nm
15
A or ?
Measure _at_ this wavelength!
Other components of the mixture
C
B reagent ? C
Compound is chemically derivitized (transformed)
prior to the measurement of absorbance
Wavelength, nm
16
Cu2
Hg2
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