Development of a Phosphate Biosensor for Soil and Ground Water [PO43-] Detection Serkan Akar Florida International University/Applied Research Center Dept. of Biomedical Engineering Master

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Development of a Phosphate Biosensor for Soil and
Ground Water PO43- DetectionSerkan
AkarFlorida International University/Applied
Research CenterDept. of Biomedical Engineering
Masters Student
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Background

• The recent research shows that stored uranium in
  Hanford causes extensive contamination in the
  soil and ground water.
• Phosphate PO43- reacts with Uranium and reduces
  it from U6 to U4. (Stabilization of Uranium)
• The detection/quantification of PO4 is
  essential.

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Method

• Develop an enzymatic biosensor which will carry
  out the reaction and produce H2O2.
• The produced H2O2 is detected via
  electro-chemical methods.
• The concentration of PO4 is then calculated
  from the calibration curve obtained as a result
  of addition of PO4 in the solution.

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Reaction

• PyrPO4O2 POX Acetyl P
  H2O2 CO2

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Detection Mechanism

• The reaction produces H2O2 which can be
  electro-chemically detected.
• H2O2 2H 2e- O2
• The Sensing Response is obtained from oxidation
  current of above reaction

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Experimental Setup
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Experimental Setup
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CV Background
Buffer Solution
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Amperometry Background
Current
Time
DCPA of The Buffer
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Graphs of H2O2
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Normalized Data
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Conclusion

• Obtained data clearly indicates that PO4 can be
  detected and measured/quantified successfully by
  the designed system.

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Acknowledgement

• Dr. Leonel Lagos (DOE Fellows Program Director)
• Dr. Vekalet Tek ( ARC Mentor)
• Dr. Thomas G. Thundat (ORNL Mentor)