empty microtiter- plate-well

1
Wash. Add sample solution containing the antigen
and a limited amount of monoclonal anti-body
add coating solution containing the antigen and
the antigen carrier protein
empty microtiter- plate-well
The secondary, enzyme-marked anti-body binds to
the primary anti-body.
Wash after competition of the free antigen and
the coating antigen for the anti-body. A relative
amount of anti-body binds to the coating of the
well
Add secondary, enzyme-marked anti-body.
P
S
Add the enzyme substrate. The enzyme, linked
to the sencondary anti-body, produces a colored
product.
The intensity of the color is relative to the
amount of antigen in the sample. Measure with a
photometer, i.e. an ELISA reader.
S
P
S
P
S