Compound Light Microscope

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Compound Light Microscope

• Has two lenses
• Light must pass through object to be seen

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Eyepiece

• Maginifies 10X
• Do Not Touch
• Clean with lens paper only

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Body tube Rotates to move eyepiece
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Revolving Nosepiece Turns to changes objectives
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High Power Objective Magnifies 40X Total
magnification 400X
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Low Power Objective Magnifies 4x Total 40X Medium
Power Magnifies 10X Total 100X
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Stage Place slide to be viewed
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Changes the amount of light passing through
specimen
Diaphragm-disc under stage
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Base Place hand under base to hold or carry
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Coarse Adjustment Moves stage to focus Never use
when on High Power
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Fine Adjustment Moves stage small amount to focus
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Arm Hold to carry microscope
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Rules for using the Microscope

• Never touch lenses
• Use lens paper only to clean lenses (eyepiece and
  objectives)
• Carry with two hands, one on arm and the other
  under the base
• Never use coarse adjustment with high power
  objective

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Important Words To Know

• Specimen-the object or organism you are going to
  look at through the microscope
• Slide-rectangle piece of glass to put your
  specimen on
• Cover glass-glass square you put over the
  specimen
• focus-to make clear
• Field of view- the circle of light you see when
  looking through the microscope

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Making a slide

• Place specimen in center of slide
• Place drop of water over specimen
• Place cover slip over specimen at an angle

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Microscope Procedure

• Start with low power
• Focus with coarse adjustment, make final
  adjustment with fine adjustment
• Center object in field of view
• Change to medium power
• Focus and center object
• Change to high power
• Focus with fine adjustment only