Aromasin? (Exemestane) in Breast Cancer

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??????????????????????????????????
??? ??? ??????????
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???(breast cancer)???????????,??????????????
????????????????????????41??,?????????????14
,???????????????
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? ???????

• ??????????????(MTHFR)??
• C677T?A1298C????????????
• ?????????
• ??????????????????, ??
• ???????????????????
• ????

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? ????
2008?1??2009?4? ?????????? ?61??????
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?????
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? ????

• ? ?????????
• ??WHO????????? ??????(CR)?????(PR)????(SD)??
  ?(PD)?

?CR?PR???
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? ???????????
0? ??????? I? ???????????????lt2/3?
?? II? ????????2/3??? III?
??????????,??????(?) ??????
?IIII????????!
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? ??????
? ????DNA???? ? MTHFR C677T ??????? ? ???? ?
Hinf I??MTHFR 677??DNA??????? ? MTHFR
A1298C??????? ? ???? ? Mbo ???MTHFR
A1298C??DNA???????
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? MTHFR C677T?????????

• ??
• 4dNTPs(?????(??)????))
• ??(?????(??)????)
• Taq ?(??????????)
• ??????Hinf I(?????(??)????)
• 10Buffer(?????(??)????)
• ??
• PCR ?????(??MJ??,??Biometra??)
• ???????(016000r/min) (??Eppendorf??)
• ??????(02µl, 0100µl, 01000µl)(GILSON??)

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• PCR??MTHFR 677????
• (198bp)
• MTHFR 677?????
• (Hinf I??PCR????)
• 
  
• ????

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? ????

• 1)??677C/C????, PCR???????????Hinf I
  ????,PCR????????,?????????198bp??,?????198bp?????
• 2)??677T/T????,PCR?????????Hinf
  I???????175bp?23bp?????????23
• bp???????????????????,??????????????175bp?????
• 3)??677C/T????,???198bp?175bp??23bp???????23bp????
  ??????????????,????????????198bp?175bp?????

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? Hinf I??MTHFR 677??DNA ???????

• lane 1 Maker
• lane 2 C/C genotype
• lane 3 C/T genotype
• lane 4 T/T genotype.

198bp
175bp
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? MTHFR A1298C?????????

• ??
• 4dNTPs(?????(??)????))
• ??(?????(??)????)
• Taq ?(??????????)
• ??????Mbo ?(?????(??)????)
• 10Buffer(?????(??)????)
• ??
• PCR ?????(??MJ??,??Biometra??)
• ???????(016000r/min) (??Eppendorf??)
• ??????(02µl, 0100µl, 01000µl)(GILSON??)

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• PCR??MTHFR A1298C????
• (84bp)
• MTHFR A1298C?????
• (Mbo???PCR????)
• 
  
• ????

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? ????

• 1)??1298A/A????,PCR?????????Mbo
  ???????56bp?28bp?????????28bp??????????????????,??
  ???????????56bp?????
• 2)??1298A/C????,???84bp?56bp??28bp???????28bp?????
  ?????????????,?????????????84bp?56bp?????

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? Mbo ???MTHFR A1298C?? DNA???????

• lane 1 Maker
• lane 2 Product of PCR
• lane 3 A/A genotype
• lane 4 A/C genotype.

84bp
56bp
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? MTHFR??????????????(?1)
Genetype C677T A1298C type T/T C/T C/C A/A A/C C/C n 18 29 14 44 17 0 Effects of chemotherapy X2 P X2 P
Genetype C677T A1298C type T/T C/T C/C A/A A/C C/C n 18 29 14 44 17 0 CRPR() SDPD() 15(83.3) 3(16.7) 21(72.4) 8(27.6) 6(42.9) 8(57.1) 31(70.5) 13(29.5) 11(64.7) 6(35.3) 0 0 X2 P X2 P
Genetype C677T A1298C type T/T C/T C/C A/A A/C C/C n 18 29 14 44 17 0 CRPR() SDPD() 15(83.3) 3(16.7) 21(72.4) 8(27.6) 6(42.9) 8(57.1) 31(70.5) 13(29.5) 11(64.7) 6(35.3) 0 0 0.72 0.40 5.54 0.02 0.19 0.67 0.72 0.40 5.54 0.02 0.19 0.67
Genetype C677T A1298C type T/T C/T C/C A/A A/C C/C CRPR() SDPD() 15(83.3) 3(16.7) 21(72.4) 8(27.6) 6(42.9) 8(57.1) 31(70.5) 13(29.5) 11(64.7) 6(35.3) 0 0
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? MTHFR????????????????? ???(?2)
Genetype C677T A1298C type T/T C/T C/C A/A A/C C/C n 18 29 14 44 17 0 reaction rate of chemotherapy X2 P X2 P
Genetype C677T A1298C type T/T C/T C/C A/A A/C C/C n 18 29 14 44 17 0 0() ?-?() 3(16.7) 15(83.3) 10(34.5) 19(65.5) 9(64.3) 5(35.7) 13(29.5) 31(70.5) 9(52.9) 8(47.1) 0 0 X2 P X2 P
Genetype C677T A1298C type T/T C/T C/C A/A A/C C/C n 18 29 14 44 17 0 0() ?-?() 3(16.7) 15(83.3) 10(34.5) 19(65.5) 9(64.3) 5(35.7) 13(29.5) 31(70.5) 9(52.9) 8(47.1) 0 0 1.72 0.19 7.38 0.01 2.86 0.09 1.72 0.19 7.38 0.01 2.86 0.09
Genetype C677T A1298C type T/T C/T C/C A/A A/C C/C 0() ?-?() 3(16.7) 15(83.3) 10(34.5) 19(65.5) 9(64.3) 5(35.7) 13(29.5) 31(70.5) 9(52.9) 8(47.1) 0 0
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?.MTHFR??????????????????
??????
5,10-MTHF
????
??????
MTHFR
DNA?? ???
? ? ?
MTHFR genovariation
5-MTHF
DNA?? ????
s-?????
???? ????
DNA????
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?.????????????
MTHFR 677 C/T IIII??????
MTHFR 677 C/T ???????
50.0ltP
50.0ltP
insignificant differences
MTHFR C677T???? ?????????????? ?????????
MTHFR 677 T/T ???????
MTHFR 677 T/T IIII??????
Plt0.05
Plt0.05
significant differences
MTHFR 677 C/C ???????
MTHFR 677 C/C IIII??????
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MTHFR 1298A/C ???????
MTHFR 1298A/C IIII??????
50.0ltP
50.0ltP
insignificant differences
MTHFR A1298C???? ?????????????? ??????
MTHFR 1298A/A ???????
MTHFR 1298A/A IIII??????
?????1298???????????????????677????, ?
1298?????????????????677????,????????
Vander Put NM, Gab reels F, Stevens EM, et al. A
second common Mutation in the
methylenetetrahydrololate reductase gene an
additional risk factor for neural-tube
defectsJ. Am J Hum Genet,2005,62(2)1044-1051.