LUCIFERASE

1
LUCIFERASE

• A. Cengiz Öztireli

2
BIOLUMINESCENCE

• BIOLUMINESCENCE (LIVING LIGHT)
• Milky Sea

3
BIOLUMINESCENCE

• Applications

4
BIOLUMINESCENCE
Sea Anemones
Bacteria
Dinoflagellates
5
BIOLUMINESCENCE

• FIREFLYES (PHOTINUS)
• Attracting mates
• Attracting prey

6
LUCIFERASE

• BACTERIA

• FIREFLY

7
BIOLUMINESCENCE

• 8 ATP ? 1 Green Photon
• Reaction

ATP
8
BIOLUMINESCENCE

• Color
• P
• P F ? F P
• Proteins around
• Structure

9
PHOTINUS PYRALIS (FIREFLY)
10
PHOTINUS PYRALIS LUCIFERASE

• Luciferase Luc-COOH

ATP
Luciferase Luc-CO-AMP
O2
Luciferase LucO
11
PHOTINUS PYRALIS LUCIFERASE

• Structure and Functioning

C-Terminal
Cleft
N-Terminal
12
CONCLUSIONS

• THANK YOU