Clinical Microbiology

1
Clinical Microbiology

• Growth Dependent ID
• Selective Media
• Differential Media
• Enrichment Culture
• Transport Media
• Blood Agar
• Chocolate Agar

2
Diagnostic Media Products

• Fermentation Media
• Diagnostic tests
• API 20E strips
• Enterotube

3
Anaerobic Cultures

• Anaerobic jars
• Reducing agents
• Anaerobic Hoods
• Safety Hoods
• BL1, BL2, BL3, BL4

4
Antimicrobial Drug Sensitivity

• MIC minimum inhibitory concentration
• Disk Diffusion
• Tube Dilution
• Antibiogram

5
Immunodiagnostics

• Antibody Titers
• Skin Tests
• Coated-particle Agglutination
• Fluorescent Antibodies

6
Monoclonal Antibody

• Polyclonal Antibodies
• Hybridoma
• Myeloma

7
Antigen-Antibody Reactions

• Neutralization
• Precipitation
• Agglutination

8
Enzyme-linked Immunosorbent Assay
ELISA Direct Antibody Bound to Well Indirect
Antigen Bound to Well Enzyme Magnify by
Repeatedly Turning Substrate to Product Colorless
Substrate Colored Product

9
Indirect ELISA
Direct ELISA
10
Immunological Tests

• ELISA uses
• HIV, Drugs, Viruses
• Radioimmunoassay
• Immunoblot

11
Nucleic Acid Based Methods
Nucleic Acid Probe Hybridization Filter or
Dipstick Reporter Polymerase Chain Reaction PCR
12
Clinical Micro Word List
Growth Dependent ID Anaerobes Selective
Media Differential Media Antimicrobial Drug
Sensitivity Immunodiagnostics Antibody Titers
Skin Test Monoclonal Antibodies Coated-Particle
Agglutination Fluorescent Antibodies Enzyme-Linked
Immunosorbent Assay ELISA
Direct Indirect Radioimmunoassay Immunoblot Nucl
eic Acid probes Polymerase chain Reaction
PCR Diagnostic Virology Electron Microscope