Chemistry in action

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Chemistry in action.Or.Ask your friendly
chemist
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Chemistry of electrophoresis and
electrolysisElectric fieldsand electric
currents
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SDS-PAGE, DNA electrophoresis and the need for
gel (sieving medium)Without a viscous medium,
all move at the same rate in electric fieldGel
structure serves to retard larger solute
particlesDNA molecules, SDS proteins have
equal charge/massElectrophoresis occurs
between the electrodes (field-driven).
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Electrolysis always occurs during
electro-phoresis.Cathode produces H2 at twice
the rate that anode produces O2Current is
carried by solute ions. Electrons arent soluble
in H2O.Example TAE buffer tris
suppliescations(),acetatesupplies
anions(-).Electrolysis occursat the electrodes.