Title: Ch. 5 Molecular Tools for Studying Genes and Gene activity
1Ch. 5 Molecular Tools for Studying Genes and Gene
activity
2DNA electrophoresis
3Gel electrophoresis (Agarose / Acryl amide)
Pulse-field gel electrophoresis
SDS-PAGE
4Two-dimensional (2D) gel electrophoresis
5Ion exchange chromatography
6Gel filtration chromatography
7Autoradiography, Densitometry, Phosphorimager
8Non-radioactive probe
9Southern blotting
10DNA fingerprinting
11DNA typing
12Northern blotting
13In situ hybridization using fluorescent probe
14DNA sequencing Sanger dideoxy method
15Automated DNA sequencing
16Restriction mapping
17Restriction mapping
18Southern blots in Restriction mapping
19Site-directed mutagenesis using PCR
20S1 mapping (5-end)
21S1 mapping (3-end)
223-end labeling by end-filling
23Primer extension
24Run-off transcription
25G-less cassette assay
26Nuclear run-on transcription
27Reporter assay (CAT assay)
28Filter binding assay
29Electrophoretic mobility shift assay (EMSA)
30DNase footprinting
31DMS footprinting
32Knockout mice embryonic stem (ES) cell stage
33Knockout mice animal stage