Ch. 5 Molecular Tools for Studying Genes and Gene activity

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Ch. 5 Molecular Tools for Studying Genes and Gene
activity
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DNA electrophoresis
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Gel electrophoresis (Agarose / Acryl amide)
Pulse-field gel electrophoresis
SDS-PAGE
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Two-dimensional (2D) gel electrophoresis
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Ion exchange chromatography
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Gel filtration chromatography
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Autoradiography, Densitometry, Phosphorimager
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Non-radioactive probe
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Southern blotting
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DNA fingerprinting
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DNA typing
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Northern blotting
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In situ hybridization using fluorescent probe
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DNA sequencing Sanger dideoxy method
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Automated DNA sequencing
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Restriction mapping
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Restriction mapping
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Southern blots in Restriction mapping
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Site-directed mutagenesis using PCR
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S1 mapping (5-end)
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S1 mapping (3-end)
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3-end labeling by end-filling
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Primer extension
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Run-off transcription
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G-less cassette assay
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Nuclear run-on transcription
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Reporter assay (CAT assay)
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Filter binding assay
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Electrophoretic mobility shift assay (EMSA)
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DNase footprinting
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DMS footprinting
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Knockout mice embryonic stem (ES) cell stage
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Knockout mice animal stage