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1
Garlic and Health, P9 report

• Liverpool, 17-21 February 2003
• INRA, Avignon, France
• V. Chovelon, research scientist
• JP Leroux, research technician
• F. Mirrione, temporary agent (1.5 month)

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Objectives

• Somatic embryogenesis, genetic characterisation
• plant regeneration
• acclimatisation and field evaluation
• sanitary aspects
• meristem culture on selected clones
• production of virus-free bulbs

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Milestones

• Embryogenesis

4
Milestones
Sanitary aspects
5
Deliverables
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Rouge Reunion

• plants from callus and cell suspensions

• In vitro culture (1)
• acclimatisation (2)
• AFLP analyses
• flow cytometry
• phenotypic notations

- 1st field evaluation at Reunion Island by P8 (3)
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Messidrôme and Morasol
plants from callus or cell suspensions
In vitro culture (2) - regeneration
- bulb maturation Acclimatisation (2) 1st
field culture (3) (4) 166 Mes cal 145 Mol cal
125 Mol ecs 153 Mes ecs 2nd field culture
(4) - 282 Messidrôme cal - 143 Morasol cal
- 145 Morasol ecs
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1st field evaluation

• notations (plant development, height, leaves
  colour, bulbs shape and weight)
• ploïdy level

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1st field evaluation
sulphur compounds concentration (HPLC)
Morasol and Messidrôme
Rouge Reunion
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1st field evaluation
sulphur compounds proportion (HPLC)
Morasol and Messidrôme
Rouge Reunion
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Printanor
plants from callus
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Sanitary aspects

• production of virus-free plants
• Messidrôme, Morasol, Printanor
• 300 plants / var. (Nov-Dec 02)
• virological control
• all the plants were potyvirus-free
• some one contained latent viruses
• Messidrôme 100 GCLV
• Morasol 83 MbFV
• Printanor 100 MbFV

13
Sanitary aspects

• virus elimination by meristem culture

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Workplan for 2003

• Embryogenesis, genetic characterisation
• Rouge Reunion
• 2nd field evaluation (P8), biochemistry (P5)
• Messidrôme, Morasol and Printanor from callus
  
• 2nd field culture and evaluation (P9)
• flow cytometry (P8), AFLP (P1), biochemistry (P5)
• Messidrôme, Morasol and Printanor from cell
  suspension
• 1st field evaluation
• flow cytometry (P8), AFLP (P1), biochemistry (P5)
• Sanitary aspects
• production of virus-free bulbs (P9)