NANOSCALE IMAGING AND SENSING RESEARCH

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NANOSCALE IMAGING AND SENSING RESEARCH
P R O J E C T O V E R V I E W
What Can We See With Quantum Dots (Q-Dots)?
Faculty Elisabeth Papazoglou, PhD, Drexel
University. E-mail esp25_at_drexel.edu
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NANOSCALE IMAGING AND SENSING RESEARCH
P R O J E C T O V E R V I E W
Absorption and Emission Spectra
Faculty Elisabeth Papazoglou, PhD, Drexel
University. E-mail esp25_at_drexel.edu
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NANOSCALE IMAGING AND SENSING RESEARCH
P R O J E C T O V E R V I E W
How Do Particles of This Size Interact With Cells?

• Valuable model system due to fluorescence can
  guide the understanding of other nanosystems.
• Two types of cell cultures
• 1. Bovine aortic endothelial cells (BAEC).
• 2. Ductal carcinoma cells cancer cells.
• Plate-coated with Q-dot solution.
• With or without collagen culture cells.
• Trypsinize centrifuge.
• Fluorescence Microscopy to see if the cells
  did in fact uptake the Q-dots.

Faculty Elisabeth Papazoglou, PhD, Drexel
University. E-mail esp25_at_drexel.edu
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NANOSCALE IMAGING AND SENSING RESEARCH
P R O J E C T O V E R V I E W
Cancer Cells and Q-dots
Current Activities

• Functionalization with Epidermal Growth Factor
• Raman Spectroscopy

Near Future

• Diffusion through Skin Reconstruct
• Encapsulation in Liposomes

Faculty Elisabeth Papazoglou, PhD, Drexel
University. E-mail esp25_at_drexel.edu
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NANOSCALE IMAGING AND SENSING RESEARCH
P R O J E C T O V E R V I E W
Q-dots As Probes of Cellular Pathways
What are the Q-dots? The quantum dots (Evident
Technologies) are semiconductor nanocrystals
(diameter 2-10 nm) made from CdSe with an
outside shell of ZnS. Their size and their
surface chemistry are the main determinants of
their optical, electronic, and chemical
properties. We used Carboxyl and Amine
terminated Qdots with diameters of 20-30 nm.
Preliminary Experiments

• Cell Types Bovine Aortic Endothelial Cells
  (BAEC) and Human Ductile
• Carcinoma Cells (HDC)
• Q-dots Lake Placid Blue (AT and CT terminated)
• Techniques Syringe loading and Q-dot coating
• Live/dead assay Calcein AM and Ethidium
  homodimer-1

Faculty Elisabeth Papazoglou, PhD, Kambiz
Pourrezaie, PhD, Bahar Edrissi, Emir Rezvan, all
at Drexel University. E-mail esp25_at_drexel.edu
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NANOSCALE IMAGING AND SENSING RESEARCH
P R O J E C T O V E R V I E W
Syringe Loading Techique
AT Versus CT Q-dots (Q-dot Coating Technique)
Faculty Elisabeth Papazoglou, PhD, Kambiz
Pourrezaie, PhD, Bahar Edrissi, Emir Rezvan, all
at Drexel University. E-mail esp25_at_drexel.edu
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NANOSCALE IMAGING AND SENSING RESEARCH
P R O J E C T O V E R V I E W
BAEC Versus HDC (Q-dot Coating Technique)
Live / Dead Assay (Q-dot Coating Technique)
Faculty Elisabeth Papazoglou, PhD, Kambiz
Pourrezaie, PhD, Bahar Edrissi, Emir Rezvan, all
at Drexel University. E-mail esp25_at_drexel.edu
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NANOSCALE IMAGING AND SENSING RESEARCH
P R O J E C T O V E R V I E W
Goals and Future Work
Key Challenges
Faculty Elisabeth Papazoglou, PhD, Kambiz
Pourrezaie, PhD, Bahar Edrissi, Emir Rezvan, all
at Drexel University. E-mail esp25_at_drexel.edu
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NANOSCALE IMAGING AND SENSING RESEARCH
P R O J E C T O V E R V I E W
Detecting Proteins With Light In Solution and In
Cells

• Proteins are much smaller (10nm) than one-half
  the wavelength of visible light (200nm), which
  is the necessary size for direct imaging.
• Solution Use light effects such as evanescent
  waves to induce amplification through surface
  plasmon resonance (SPR), fluorescence, and FRET.

Faculty Elisabeth Papazoglou, PhD, Kambiz
Pourrezaei, PhD, Drexel University. E-mail
esp25_at_drexel.edu
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NANOSCALE IMAGING AND SENSING RESEARCH
P R O J E C T O V E R V I E W
Nanoprobe Operation
Faculty Elisabeth Papazoglou, PhD, Kambiz
Pourrezaei, PhD, Drexel University. E-mail
esp25_at_drexel.edu
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NANOSCALE IMAGING AND SENSING RESEARCH
P R O J E C T O V E R V I E W
Nanoprobe Setup
Faculty Elisabeth Papazoglou, PhD, Kambiz
Pourrezaei, PhD, Drexel University. E-mail
esp25_at_drexel.edu
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NANOSCALE IMAGING AND SENSING RESEARCH
P R O J E C T O V E R V I E W
Nanoprobe Advantages
Faculty Elisabeth Papazoglou, PhD, Kambiz
Pourrezaei, PhD, Drexel University. E-mail
esp25_at_drexel.edu
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NANOSCALE IMAGING AND SENSING RESEARCH
P R O J E C T O V E R V I E W
Antibody Functionalization of Silica Surface
Faculty Elisabeth Papazoglou, PhD, Kambiz
Pourrezaei, PhD, Drexel University. E-mail
esp25_at_drexel.edu
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NANOSCALE IMAGING AND SENSING RESEARCH
P R O J E C T O V E R V I E W
Preliminary Cell Viability
Faculty Elisabeth Papazoglou, PhD, Kambiz
Pourrezaei, PhD, Drexel University. E-mail
esp25_at_drexel.edu
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NANOSCALE IMAGING AND SENSING RESEARCH
P R O J E C T O V E R V I E W
Alternative Detection Schemes
Faculty Elisabeth Papazoglou, PhD, Kambiz
Pourrezaei, PhD, Drexel University. E-mail
esp25_at_drexel.edu
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NANOSCALE IMAGING AND SENSING RESEARCH
P R O J E C T O V E R V I E W
Evanescent Waves Induce SPR
Faculty Elisabeth Papazoglou, PhD, Kambiz
Pourrezaei, PhD, Drexel University. E-mail
esp25_at_drexel.edu
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NANOSCALE IMAGING AND SENSING RESEARCH
P R O J E C T O V E R V I E W
Surface Enhanced Raman of Zinc Oxide Powder
Faculty Elisabeth Papazoglou, PhD, Kambiz
Pourrezaei, PhD, Drexel University. E-mail
esp25_at_drexel.edu
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NANOSCALE IMAGING AND SENSING RESEARCH
P R O J E C T O V E R V I E W
Surface Enhanced Raman of Zinc Oxide Powder
Faculty Elisabeth Papazoglou, PhD, Kambiz
Pourrezaei, PhD, Drexel University. E-mail
esp25_at_drexel.edu
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NANOSCALE IMAGING AND SENSING RESEARCH
P R O J E C T O V E R V I E W
SurfaceEnhanced Raman for Phosphate Buffer Saline
Solution
Faculty Elisabeth Papazoglou, PhD, Kambiz
Pourrezaei, PhD, Drexel University. E-mail
esp25_at_drexel.edu