?Capillary Electrophoresis? PowerPoint PPT Presentation

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Title: ?Capillary Electrophoresis?


1
?????
  • ?Capillary Electrophoresis?

2
? ?
?? ?electrophoresis? ?????????????????????????
????? ?capillary electrophoresis?
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3
? ?
?????Tiselius???????,??????????????5?????,??????
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4
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  • ???????

? ?eE
? ????????? ?e??????? E???????????????????
??????????,????????????,??????????????????????????
??????????,???????????????????????????????????????
?,??????????????
??-??
?????!!!
5
  • ?????

?1? ???????
? ?eE ?eU/L
U????????????? L???????? ??????????????????????
?2? ?????????
N ?eU/2D
???????????????,?????????????????? ????????,??????
?????500 V? ???????,?????20,000
60,000 V?????
6
?3? ???
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7
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8
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????,???????????????,???????????????????

??????????????????????????????????????,?????
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9
?????????????
  • ??????
  • ?capillary zone electrophoresis, CZE?
  • ???????
  • ?capillary gel electrophoresis, CGE?
  • ???????
  • ?capillary isota- chophoresis, CITP?
  • ???????
  • ?capillary isoelectric focus, CIEF?

10
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11
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???????????,DNA??,????????????????????? ??????????
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12
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13
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?????????????????pH?????????????????????????????,?
???,?????? pI, ???????pH??????pH????????????,?????
????????pH?,??????????,????????????
???pH?,??????????????pH??????,???????????????,????
????????????pI???,??????,????? ??????pH????,????pH
????pI????,???????,?????????????,?????????????????
?,??????????
14
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  • ??????????????????????????????????????????????????
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15
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