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?Capillary Electrophoresis?

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Title: ?Capillary Electrophoresis?


1
?????
  • ?Capillary Electrophoresis?

2
? ?
?? ?electrophoresis? ?????????????????????????
????? ?capillary electrophoresis?
?????????????????????????????,????????????????????
?????????
?????? ???????????????????????????,???????????????
????????,????????
3
? ?
?????Tiselius???????,??????????????5?????,??????
?????????
4
??????????
  • ???????

? ?eE
? ????????? ?e??????? E???????????????????
??????????,????????????,??????????????????????????
??????????,???????????????????????????????????????
?,??????????????
??-??
?????!!!
5
  • ?????

?1? ???????
? ?eE ?eU/L
U????????????? L???????? ??????????????????????
?2? ?????????
N ?eU/2D
???????????????,?????????????????? ????????,??????
?????500 V? ???????,?????20,000
60,000 V?????
6
?3? ???
????????????????,??????????????????????,??????????
???,???????????????????????????????????
7
???????
8
?????????????????? ????????????????????????????,??
????,???????????????,???????????????????

??????????????????????????????????????,?????
???????
9
?????????????
  • ??????
  • ?capillary zone electrophoresis, CZE?
  • ???????
  • ?capillary gel electrophoresis, CGE?
  • ???????
  • ?capillary isota- chophoresis, CITP?
  • ???????
  • ?capillary isoelectric focus, CIEF?

10
??????
??????????????,????????????????????,?????,???,????
???
11
???????
???????????,DNA??,????????????????????? ??????????
???????????,??????????????????????,???????????????
?????????????????????????????????????,????????????
?????
12
???????
??????????????????????????????????????????????,???
????????????????????????????????????????????,?????
??????,??????????,????????????????????????,???????
?????,????????????????????????????????????????????
???????????????????,???????,????????,?????????????
??????????
13
???????
?????????????????pH?????????????????????????????,?
???,?????? pI, ???????pH??????pH????????????,?????
????????pH?,??????????,????????????
???pH?,??????????????pH??????,???????????????,????
????????????pI???,??????,????? ??????pH????,????pH
????pI????,???????,?????????????,?????????????????
?,??????????
14
??????
  • ??????????????????????????????????????????????????
    ????????????,???????????????
  • ??????????????????????????????????????????????????
    ???,??????????????????????????????????????????????
    ???????????

15
??????????
  • ???????,????????????
  • ???????,????????????,???????????????????????,?????
    ?????????????????????????
  • ??????????????,??????????,???????????????????????
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