Live food aquaculture training course

1
Demonstration of Artemia decapsulation
2
Background

• Alveolar layer of the cyst are removed

3
Why decapsulated cysts?

• A complete contamination free and sterile product
• Separation problems after hatch are avoided

4
Preparation
Addition of water and strong aeration
Tank set-up with stainless steel filter and
aeration system
5
Hydration

• Dry cysts are hydrated in water with strong
  aeration

6
Addition of chlorine and caustic soda gt
increases pH above 10
Temperature should be maintained within 25-30?C

• Addition of chlorine and hypoclorite solution

7
Colour change
Process lasts from 5-15 min

• Dark brown
• Grey
• Orange
• The process is stopped immediately after orange
  colour appear

8
Checking of bouyancy

• Decapsulated cysts will sink
• Non-decapsulated cysts will float

9
Termination of process

• Process is stopped by adding large amounts of
  water until the smell of chlorine has disappeared

• Hypoclorite residue absorbed by the cysts is
  neutralized by
• 0.1 Sodium Thio-sulfate

10
Transfer to hatching tank