Lecture 12 HPLC

1
Lecture 12HPLC
2
Lecture 12HPLC
3
GC setup
4
LC setup
5
LC Injector
6
Back to Van Deempter
A
8
H
C?
7
Back to Van Deempter
dp2?

8
Ce dp
H
?
DMP
8
Mobile Phase
dp2?

8
Ce dp
H
?
DMP
9
LC Columns Particle Size (dp)
dp2?

8
Ce dp
H
?
DMP
Dong, M. Todays Chemist at Work. 2000, 9(2),
46-48.
10
LC Columns Particle Size (dp)
dp2?

8
Ce dp
H
?
DMP
11
LC Columns Tortuosity (g)
dp2?

8
Ce dp
H
?
DMP
Ascentis Express, Supelco, technical information
12
Injector/column interaction
13
Types of LC columns
C8
C18
14
Column elution
15
Size exclusion chromatography