Title: Anti-HBc detection in ULTRIO not-repeatable reactive blood donors
1Anti-HBc detection in ULTRIO not-repeatable
reactive blood donors
- Piotr Grabarczyk, Ewa Brojer
- Institute of Haematology and Transfusion
Medicine, Poland
2HBV DNA screening in Poland
- Obligatory HBV DNA screening in all blood donors
since January 2005 - PCR Cobas in pools of 24 donations (since 2007 in
pools of 6) - TMA ULTRIO in single donations
3ULTRIO TMA not-repeatable reactives
- DEFINITION
- Triplex ()
- Discrimination HBV (-) HCV (-) HIV(-)
- VERIFICATION METHODS
- alternative NAT tests (Cobas, Arthus)
- HCV RNA(-), HIV RNA(-) and HBV DNA(-) false
positive triplex assay - HBV DNA positive testing for anti-HBc to
distinguish occult and window period infection
4S/co TMA value and anti-HBc detection in samples
reactive in ULTRIO assay(2005)
250 191 donations screened 9 positives
(triplex/dHBV) 129 not-repeatable
S/co of ULTRIOgt10
reactive (0.05)
12 HBV DNA ()
in PCR (10) 5/9 (56) anti HBc
positive
S/co of ULTRIOgt10
10/12 (83,3) anti-HBc positive
5Aim of the study (2006)
- Analysis of S/Co distribution in samples with not
-repeatable results of ULTRIO TMA - Assessment of anti-HBc frequency in all samples
with not-repeatable results in ULTRIO TMA
6HBV DNA screening yield in 2006
- 247 263 donations
- 1 positive
366 not-repeatable
reactive (0,14) - 3 (0,8)HBV DNA positive 363
negative in - in PCR
PCR
S/co of TMAgt10
7S/co of ULTRIO test in 363 HBV DNA negative
not-repeatable samples
S/co
8Anti-HBc frequency in HBV DNA negative samples
with not-repeatable results of ULTRIO test
dependence on S/co value
Anti-HBc frequency in donors negative in TMA
assay 7
ULTRIO S/co value
9Instead of conclusions
- Are the anti-HBc positive donors with
not-repeatable ULTRIO results infected with
occult HBV with virus load below sensitivity of
the assay? - What is the clinical significance of such
infections? - Can anti-HBc testing be useful in confirmatory
procedure?