?????????????-????(SAP?)

1
?????????????-????(SAP?)

• ?????????

2
????

• 1??????????
• 2??????(????????)
• 3?SAP?????
• 4??????
• 5? 0.01M PBS(pH7.27.6)
• 6?BCIP/NBT????
• 7??????

3
????
ABC?
SAP?
4
???????

• 1?????????,????,PBS??2min(????
• ????,???????)?
• 2?10???????,????10min
• 3?????,??,??????????????????
• ??,37???45min? PBS??,33min
• 4???????????IgG???,37???20min
• 5? PBS??,33min
• 6??????????????????,37??????
• 20min? PBS??,33min
• 7??????(BCIP/NBT)
• 8????????,???

5
??

• ??????????????????,?????????

6
??????? HRP---?????---?? ??Epos?
Enhanced polymer one-step staining
7
????
??
HRP
???????
EPOS ??
8
????????????? ???????,???
????HRP,???? ????????,??
HRP---?????---?? ????
9
????? ????,??,????, ????
????????, ????
10
??EnVision?(?PowerVison) Enhanced
Labelled Polymer System ??HRP ---?????---?? ??
11
????
??
??
?
???????
???
???
PowerVision??
12
?? ????(?????????70? HRP???10 ?????)
???? ????????Avidin?Biotin????
13
??????
1. ???? 2.?????? ??? 3. ??????
4. ???? 5. ??????? 6. ??
14

• ????
• 1?????????,PBS?33min
• 2?3H2O2????10min,?????????
• ?????????,PBS??5min
• 3??????????????????, 37?
• ??1hr
• 4? PBS??,33min
• 5? ??HRP??????Ig???,37?????
• ?2030min
• 6? PBS??,33min
• 7??????(AEC?DAB)
• 8????????,???

15
????????????????,??????????