A Carbon Nanotube

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A Carbon Nanotube Based Hydrogen
Peroxide Biosensor
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??????????H2O2????????

• ??????
• ????????

3
??

• ???????
• ?????
• ????
• ?????
• ??
• ???
• ??

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???????
??????????????????,???? (?????,?????)??????????? ?
?????????????????????? ??????????(CNTs),??H2O2?? ?
???????,??????????????? ????????
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????(CNTs)?CNTs??????????
??Lijima?1991???CNTs??,CNTs?????????? ?????????,?
??????,???????,??? ????????,??????????????????,? ?
????CNTs??????????(SWNTs)?????? ?(MWNTs)?
MWNTs
SWNTs
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????(CNTs)?CNTs??????????
??CNTs?????????,???????,?????????????????(????????
)???CNTs????????????????????? ?3???????CNTs???????
???10????
References 1. K. Balasubramanian, M. Burghard.
J. Small, 2005,1(2)180-192. 2. E. Katz, I.
Willner. J. ChemPhysChem, 2004,
5(8)1084-1104. 3. J. Wang. J. Electroanalysis,
2005, 17(1)7-14.
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H2O2??????????
GOD(OX)glucoseH2O?GOD(RED) gluconic acid H2O2
L-lactateO2 LOD(OX)?pyruvateH2O2LOD(RED)
H2O2HRP?H2OHRP-I
?????????????????????,?? ????????????H2O2,????H2O2
?? ?,?????????????????????? ??????????????????????
?? ??
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?????

• ??????CHI660a?????????,???????,?????????,????????,
  Ag/AgCl???????
• ????(SEM)??????????????????????(??JSM-6330F),????
  (TEM)???????????????????(??JEM-2010HR)?????????Br
  uker???????????-????????(??EQUINOX 55)???
• HRP?????????????? ,??gt250units/mg
• ???????????, ??????0.05mol/L pH6.5 PBS????

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????????
1 ??Nafion(?????????)?????? (GC)? 2
????????????????Thi(??,?? ?????,????????)??????
?? 3 ??????CHIT(???,??????????)
????MWNTs?HRP????Thi????H2O2 ????
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????????
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Structure
Raw material of Chitosan
Chitosan
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Structure
Nafion
Thionine
Methylene Blue
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?????

• MWNTs?????
• ????H2O2???
• ????
• ??????

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?1 MWNTs?????
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?2 MWNTs?????
H2O ?CHIT?MWNTs ???????
MWNTs-COOH?IR?
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?3 ????H2O2???CV??
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????

• ????????
• ??????
• pH??
• ??????
• ?????

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?4 ??????????????(n3)
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?5 ??????????
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?6 pH???????? (CH2O20.1mmol/L, n6)
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?7 ???????(CH2O20.1mmol/L, n6)
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?8 ?????(CH2O20.1mmol/L, n6)
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?????

• ????????????
• ????????
• ????????(?????30µmol/L5.5mmol/L
  ?????0.9995??S/N3????,????19µmol/L )
• ????(?????1.93mmol/L )
• ?????????(????????6.3,?????? )

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?9 ????????????(CH2O20.5mmol/L)
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????????????
????IAgtgtIBIC?????????????? ??????????? 1.
HRP????????MWNTs??? 2. MWNTs?????????????????HRP?
???? 3. MWNTs???????????H2O2?????? 4.
MWNTs ?H2O2???????
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?????? ???
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?10 ????????(CH2O20.5mmol/L)
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?11????????
1E-4M per step
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?12 ??????????
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?13 ???????(CH2O20.1mmol/L, n6)
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??

• ????????????????,????????
• CNTs??????????????????????
• ???????????????????

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???????

• ????Thi??????CNTs?????
• ????????CNTs?????????HRP,??????????,??????????????
  ?????????
• ??CNTs??????????,???CNTs??????????????

recognition
HRP
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????2004??????????????
???????
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Thanks