Histology for Pathology Hematopoietic Elements

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Histology for PathologyHematopoietic Elements

• Theresa Kristopaitis, MD
• Associate Professor
• Director of Mechanisms of Human Disease
• Kelli A. Hutchens, MD, FCAP
• Assistant Professor
• Assistant Director of Mechanisms of Human Disease
• Loyola Stritch School of Medicine

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Objectives

• On a peripheral blood smear identify the
  following cells erythrocytes, platelets,
  neutrophils, eosinophils, basophils, lymphocytes
  and monocytes.
• On a peripheral blood smear identify neutrophil
  band cells (also known as stab cells) and state
  the clinical significance of their presence.
• On HE stained sections, identify neutrophils,
  eosinophils, lymphocytes.
• On HE sections identify plasma cells.
• On a stained section identify the 3 major
  components of bone marrow (bone trabeculae,
  hematopoietic cells, adipose tissue).
• List the sequence of development of a
  granulocytes focusing on the neutrophil.
• On a section of bone marrow, identify a
  megakaryocyte.
• Define blast cell.

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Granulocytes Peripheral Blood Smear
Neutrophil
Eosinophil
Basophil
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Agranulocytes Peripheral Blood Smear
Lymphocyte
Monocyte
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Peripheral Blood Smear
Red blood cells
Platelets
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HE Stained Sections
Eosinophils
Neutrophils
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HE Stained Section
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HE Section Plasma Cells
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Bone Marrow
Bone
Hematopoietic cells
Adipose (fat) cells
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Based on this representative section of normal
bone marrow, what is the age the patient?
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Granulocytopoiesis
F
E
B
C
A
G
Neutrophilic series A Myeloblast B Myelocyte
(large cell, rounded nucleus) C Late
myelocyte or early metamyelocyte (nucleus
beginning to indent) E Metamyelocyte (indented
nucleus) G Band cell (much thinner nucleus) F
Segmented (mature) neutrophil
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Megakaryocyte(Mega Giant)Fragments of
cytoplasm break off to become platelets