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The Eukaryotic Cell Cycle molecules,
mechanisms, modules and mathematical models
Béla Novák Molecular Network Dynamics Research
Group Hungarian Academy of Sciences and Budapest
University of Technology and Economics
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The central dogma
DNA
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Beyond the central dogma
DNA
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Hanahan Weinberg, 2000
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The cell cycle
The cell cycle is the sequence of events
whereby a growing cell replicates all its
components and divides them more-or-less
evenly between two daughter cells ...
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exit
Cdk
G2/M
CycB
Start
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Cdk
CycB
Start
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Cdk
G2/M
CycB
Start
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CycB
AA
Cdk
Cdk
CycB
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Regulatory feedback loops
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CycB
AA
Cdk
Cdk
CycB
inactive
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?
fission yeast
budding yeast
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Mutants in Fission Yeast
gene viability trait cdc2- no block
in G1 and G2 cdc13- no endoreplication rum1-
yes sterile ste9- yes sterile wee1- yes sm
all cells cdc25- no block in
G2 cdc2op yes wt cdc13op yes wt rum1op no
endoreplication ste9op no endoreplication wee
1op yes large cells cdc25op yes small
cells wee1- rum1? no extremely small wee1-
cdc25? yes quantized cycles wee1-
cdc25op no mitotic catastrophe
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cycle time
doubling time (tD)
dm/dt ? m where ? ln2/tD
CycB
S/G2
G1
M
M
rate mass
time (min)
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Size control over the cell cycle
mass
dm/dt ? m where ? ln2/tD
CycB
S/G2
G1
M
M
rate mass
time (min)
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Inside view ?
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CycB
AA
Cdk
Cdk
CycB
G2/M
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Bifurcation diagram of the cell cycle control
network
stable s.s. unstable s.s. min/max
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M
Cdk/CycB
S/G2
G1
cell mass
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Cell cycle regulation on the bifurcation diagram
stable s.s. unstable s.s. min/max
?
M
Cdk/CycB
Exit
G2/M
S/G2
Start
G1
cell mass
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Checkpoint controls
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Homepage http//www.cellcycle.bme.hu/
Supporting agencies
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Modelling the cell division cycle
Béla Novák Molecular Network Dynamics Research
Group Hungarian Academy of Sciences and Budapest
University of Technology and Economics