Pr

1
Nanocoatings of inorganic surfaces by molecular
biomimetic
C. VREULS, A. GENIN, G. ZOCCHI, G. GARITTE,C.
ARCHAMBEAU, C. VAN DE WEERDT, J. MARTIAL
BMGG-CERM-ArcelorMittal Research Licge
Director J. MARTIALJ.Martial_at_ulg.ac.be
Aim of the Biocoat project (PPP, RW) To develop
new functionalities with high added value on
inorganic substrates through multifunctional
molecular coatings following a multidisciplinary
biomimetic approach
Strategies for surface coating Bottom-up
hierarchic assembly using engineered proteins
Interests of GEPIs high specificity for-
chemical composition - structuration (powder,
surface) - cristallographic form
GEPI Genetically Engineerd Polypeptide for
Inorganic
small peptide that recognizes and specifically
binds an inorganic surface
Inorganic-binding peptides as tools for surface
quality control GEPI applications
Nature
Molecular biomimetic
COPY NATURE
Patterned substrate 50-nm layer of Ag on pure
zinc
ZnO-binding peptide with FITC at the N-terminal
Recognition gt binding protein - inorganic
material
GEPI Linker betweeninorganic-organic materials
1st step isolate GEPI to gather incompatible
materials
2 types of galvanized steel
Selection of GEPI by Phage Display technology
Galva Gi206031 Galva DRX51
Control quality of raw or coated inorganic
surface by fluorescence analyses
Perspectives Use Gepi for powder sorting, Surface
fonctionalization
1 Librairy presentation 2 Wash 3 Elution
4 Amplification 5 next round of selection 6
sequencing after 3-4 rounds of selection
Teams Publications Patent ApplicationsAll-in-o
ne strategy for the fabrication of antimicrobial
biomimetic films on stainless steel. J. Mat.
Chem. 2009 19 4117-4125. Inorganic-binding
peptides as tools for surface quality control J.
Inorganic Biochem. 2010Plasma deposition of
antimicrobial peptides onto stainless steel for
the prevention of bacterial biofilms (submitted
to J. Mat. Chem) Biomolecules in multilayer film
for antimicrobial and easy-cleaning stainless
steel surface applications (submitted to
Biofouling) Multifunctional Coatings WO
2009/147007 A2Inorganic-binding peptides as
tools for surfaces quality control detection
PCT/EP2009/004876).
Acknowledgments The research was partly
supported by the Walloon Region (PPP program
BIOCOAT). We thank the BIOCOAT team members for
their contribution, in particular Fabrice Farina
(ArcelorMittal Research Industry, Liege, Belgium)
for providing the various inorganic material
coupons, GIGA-R, Systems Biology and Chemical
Biology Unit (ULg)