The Antibiotic Sensitivity Test

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The Antibiotic Sensitivity Test

• Presented by
• Marian Mikhail
• Undergraduate student health and science major
  Tennessee Tech University
• Cookeville, Tennessee 38505

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Project Summary

• Problem Susceptibility or resistance of bacteria
  to antibiotics
• Objective to show Antibiotics sensitivity test
  (disc diffusion method) importance in determining
  the proper antibiotics treatment in different
  species in enterobacteriaceae family.
• Hypothesis different species in the same
  bacterial family like the enterobacteriaceae
  family (gram negative rods) will have different
  susceptibility or resistance to antibiotics
• Null hypothesis different species in the same
  bacterial family like the enterobacteriaceae
  family (gram negative rods) will have the same
  susceptibility or resistance to antibiotics
• Goals and results Determination the zone of
  inhibition around each bacteria we will be able
  to determine the proper antibiotics treatment

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Introduction/Background

• problem susceptibly or resistance of bacteria
  to antibiotics
• 
  
  
  
  
  
  
  
  to screen salmonella typhi in asymptomatic
  typhoid and to find out drug resistance and
  ability of strains to transmit drug resistance to
  other bacteria (senthilkumar and prabakaran
  2005).
• Determine the susceptibility of an opportunistic
  pathogen to antimicrobial agent (Goss 2007).

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Objectives/hypothesis plus null statement)

• Objective to show Antibiotics sensitivity test
  (disc diffusion method) importance in determining
  the proper antibiotics treatment in different
  species in enterobacteriaceae family.
• Hypothesis different species in the same
  bacterial family like the enterobacteriaceae
  family (gram negative rods) will have different
  susceptibility and resistance to antibiotics
• Null hypothesis different species in the same
  bacterial family like the enterobacteriaceae
  family (gram negative rods) will have the same
  susceptibility and resistance to antibiotics

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Methods and materials

• Materials antibiotics plates-antibiotics
  different species of bacteria in the
  enterobacteriacae family-proper antiseptic
  technique.
• Experimental design
• - Disc diffusion method (Goss 2007).
• data form
• Statistics
• - by taking an average of the zone of inhibition
  and calculating the standard deviation
• -showing statistics of the most suitable
  antibiotics for treatment of diseases caused by
  enterobacteriace family

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Expected results and benefits

• Formation of zone of inhibition
• Measuring the zone of inhibition
• The greater the inhibition zone that means that
  the bacteria are susceptible to antibiotics
• The lesser the inhibition zone that means that
  bacteria are resistant to antibiotics
• If no zone of inhibition is formed that means
  that the bacteria are resistant to antibiotics
• The benefits if the bacteria are susceptible to
  antibiotics that means that the antibiotics are
  able to kill bacteria

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Project timeline
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project budget

• Budget

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I would like to thank you for your attention