cd rom part 2

1
COMPENSATION
FITC
FL1

detector
FITC -
PE
gain1
-
FL2-FL1
FITC
FL2
PE

PE -
FITC
gain1
detector
-
2
COMPENSATION
FITC
FL1

detector
FITC -
PE
gain1
-
PE
FL2
PE

PE -
FITC
gain1
detector
FL1-FL2
-
3
Uncompensated vs Compensated
FL2
FL1
4
COMPENSATION IS INTENSITY DEPENDENT
FL2
fully compensated
uncompensated
partially compensated
FL1
5
COMPENSATE INSTRUMENT USING STAINED CELLS
1. Adjust PMT voltages using unstained cells
2. Adjust compensation for each fluorochrome
6
Lymphogated Cells for Four Color Single Laser
Compensation
B
A
SSC
SSC
0
256
512
768
1024
0
256
512
768
1024
FSC -gt
FSC -gt
FSC
FSC
030695001
030695001
7
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8
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9
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10
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11
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12
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13
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14
R3
R1
R2
R4
R6
R5
15
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16
A
C
R3
B
D
R3