Laboratory Equipment

1
Laboratory Equipment

• Lab Procedures VTHT 1202

2
Centrifuge

• Used to separate substances of different
  densities that are in a solution.
• Supernatant the liquid component of a sample.
• Sediment the solid component of a sample
• Different types of centrifuges are used in
  veterinary medicine.

3
Centrifuge Types
Angled Head Most Common
Horizontal (Swing-Arm)
Microhematocrit
4
Centrifuged Blood Sample
5
Microhematocrit Tubes
6
Blood Collection Tubes

• Different types of tubes are used depending on
  what type of sample is desired.
• Whole Blood- Purple (lavendar) top tube or green
  top tubes

7
Blood Collection Tubes Continued

• When looking at serum or other factors, use
  different type of tubes.
• Oxalate Tubes GREY TOP

8
Blood Tubes Continued

• Citrate Tubes BLUE TOP

9
ACD (acid citrate dextrose)

• Used in blood collection for transfusions.
• Blood is stored in ACD vials or bottles and given
  to anemic patients.

10
Blood Collection Equipment

• Use the largest gauge of needle that is
  comfortable for animal.
• As gauge size increases, needle diameter
  decreases.

11
Blood Collection Equipment Continued

• Syringes should be picked based on one closest to
  desired volume of blood.
• A larger syringe may collapse the vessel.

12
Syringe Sizes

• 1cc Tuberculin

3cc
6cc
13
Blood Collection Continued

• Vacutainer allows blood to be collected directly
  into a blood collection tube.

14
Refractometer

• Total solids meter.
• Used to measure the refractive index of a
  solution.
• Refraction is the bending of the light rays as
  the pass from one medium into another.
• Refractometer in vet med is used to measure
  specific gravity of urine and plasma protein
  levels.
• Know how to calibrate refractometer

15
Blood Chemistry Analyzers/ CBC Machines

• Generates data on organ function and complete
  blood counts.
• Machines count cells based on size of cells in a
  predetermined sample.

16
Vet Test -IDEXX

• Chemistry Analyzer

17
(No Transcript)
18
Incubators/ Agar plates

• Used in microbiological tests.
• Sustains temperature settings (37 C)
• Agar plates are inoculated with potential
  bacteria.
• Plates are smeared with inoculation loops.

Curettes
Incubator
Culture Plates
19
Pipettes

• Gives the ability to transfer liquids from one
  place to another.
• May be plastic or glass

20
Culture Swabs
21
Slides

• Thin sheet of glass used to hold objects for
  examination under a microscope.
• Usually place specimen in center of slide to
  view.

22
Cover Slips

• Smaller, sheet of glass that may cover specimen.
• Purposes are
• To protect microscopes objective lens from
  contacting the specimen
• Creates an even thickness for viewing.

23
Washing glassware

• Wash with soap, detergent, or cleaning powder.
• Wash with hot water.
• Wash as quickly as possible after use. If a
  thorough cleaning is not possible immediately,
  put glassware to soak in water.
• All parts of glassware should be thoroughly
  scrubbed with a brush.
• Use brushes that will not scratch or abrade the
  glass surface.

24
Drying and Storing Glassware

• If glassware is to be dried, allow it to drain or
  use paper towels.
• Never tap glassware against the side of the sink!
• Dry labware by
• Hanging on wooden pegs or placing them in baskets
  with mouths downward allowing them to dry in the
  air
• Protect glassware from dust by plugging mouth and
  storing in a dust free cabinet.

25
Technician Role Re Lab Equipment

• Technician should have a thorough knowledge of
  all in-house laboratory equipment.
• Have lab manufacturers phone numbers readily
  available for assistance.
• Know how to operate laboratory instruments.
• Know how to clean appropriately.
• Know requirements, calibration measures, etc.

26
Sharps Container

• Always use an approved Sharps container to
  dispose of contaminated sharps.
• When 2/3 full dispose in compliance with
  Hazardous Waste Management Plan.

27
Recall the Metric System
28
Rules of Lab

• 1. NO FOOD OR DRINKS!!!
• Listen to instructions.
• No horseplay
• Do not handle contact lenses
• 5. Dispose of or clean all used instruments and
  slides accordingly.
• 6. Use sharps containers
• 7. Make sure that workstations are neat and
  clean.
• 8. Make sure to wear appropriate attire and PPE
  when told.

29
Watch Safety Video

• http//www.youtube.com/watch?vaEjVSMeFHqsfeature
  related