Diapositive 1

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Presentation Agar COMPASS cc Method
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The Agar COMPASS cc method allows the
enumeration of E. coli and coliforms in 24 hours
in drinking water. This method is certified by
AFNOR certification in relation to reference
method ISO EN 9308-1.
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COMPASS cc Agar
Water sample (100 ou 250 ml)
BLUE VIOLET colonies E. coli
Place the membrane front grid to the top
ß-galactosidase ß-D-glucuronidase
PINK colonies Coliforms
ß-galactosidase ß-D-glucuronidase -
Filtration
PLATING 24 HEURES 3H AT 36 2C
Enumeration without confirmation
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Simplified protocol COMPASS cc Agar
Membrane filtration
Reference method NF EN ISO 9308-1 2000
Membrane filtration
Standard test
Quick test
Tergitol 7 Agar
Agar TSA
COMPASS cc Agar
¹ 21 3 h at 36 2 C
¹ 4 h à 5 h at 36 2 C
or 44 4 h 1
¹ 24 3 h at 36 2 C
Agar TBA
Yellow colonies, lactose
¹ 19 h at 20 h at 44 0,5 C
Blue and pink colonies
Confirmation
Indole test
E. coli and coliforms enumeration
¹ 10 at 30 min under UV lamp
Tryptic soy-agar
Tryptophane broth
Without confirmation
¹ 21 2 h at 36 2 C
¹ 21 3 h at 44 0,5 C
Red colonies
E. coli enumeration
Oxidase test
Indole test
Coliforms Test (-)
E. coli Test ()
Total Coliforms enumeration
at
J3
J2
1 The incubation duration up to (44 4) h may
increase the test sensitivity
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COMPASS cc Method

• Specific detection of E. coli plus coliform
  differentiation.
• Good visual distinction between BLUE VIOLET
  colonies (E. coli) and PINK colonies (coliforms).
• Expression of 2 specific enzyme activities
  ß-galactosidase and ß-D-glucuronidase.
• Total inhibition of secondary flora.

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COMPASS cc Method

• 2 tests in 1 package a quick and easy-to-use
  simultaneous enumeration of E. coli and
  coliforms.
• Use of a single media with no confirmation.
• Results in 24 hours by direct plating.
• Time saving in relation to the 2 day reference
  method.

Filtration
PLATING 24 HEURES 3H AT 36 2C
Enumeration Without confirmation
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COMPASS cc Agar
Escherichia coli Characteristic colony
Blue-violet color
Other coliforms Characteristic colony Pink color