Lehrstuhl f

1

Internship Directed evolution of Perhydrolase
for improved perhydrolytic activity

• Lehrstuhl für Biotechnologie
• RWTH Aachen University
• Supervisors
• Prof Dr. U. Schwaneberg
• Dragana Despotovic

2
Techniques included in training

• Molecular biology techniques
• PCR ampilification and purification of PCR
  product
• Mutagenesis methods (epPCR, Site Saturation
  Mutagenesis/Site Directed Mutagenesis)
• Ligase independent cloning PLICing
• Megawhop cloning
• Transformation of E. coli and B. subtilis strains
• Biochemical methods
• Expression of Perhydrolase in B. subtilis
• Screening determination of perhydrolytic and
  esterolytic activity and finding improved
  variants
• Purification of Perhydrolase
• Electrophoresis techniques SDS electrophoresis
  and Experion system