GENETICAL FEATURES OF CHROMOSOMAL FORM

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GENETICAL FEATURES OF CHROMOSOMAL FORM C OF
MICROTUS MAXIMOWICZII FROM THE RUSSIAN FAR EAST

• Irina V. Kartavtseva, Irina N. Sheremetyeva
• Institute of Biology Soil Science, Far Eastern
  Branch of Russian Academy of Sciences,
  Vladivostok (Russia)
• Anastasia B. Ryabkova
• Khabarovsk Antiplague Station, (Russia)
• Svetlana A. Romanenko, Olga L. Gladkikh
• Institute of Molecular and Cellular Biology SB
  RAS (Russia)
• Lubov V. Frisman
• Institute of Complex Analysis of Regional
  Problems Far Eastern Branch of Russian Academy of
  Sciences, Birobidzhan (Russia)

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The role of chromosomal rearrangements in
speciation still unclearly.
Introduction
The maximowicz's vole Microtus maximowiczii
(Rodentia) is convenient species for
investigating this problem.
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All rearrangements for this species can be
divided into three types
1 - is fusion of two middle sized pairs of
metacentrics to formating of large metacentric.
2 - is Robertsonian rearrangements.
3 - is pericentric inversions of 4 pairs.

• Rearrangements by Mejer et al., 1996

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Areal and terra typica points of M. maximowiczii
Russia
Mongolia
China
Korea

• M. m. maximowiczii Schrenck
  1858,
• Amurskii and Khabarovskii regions in Russia, and
  in northeastern China
• M. m. ungurensis Kastchenko
  1913,
• Buryatia and Chitinskii regions in Russia and
  North-Eastern Mongolia

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For this species was described five chromosomal
forms (Kartavtseva et al., 2008)

• A 2n 39 - 44, NF 53 - 60 (Buryatiya),
• B 2n 38 - 42, NF 54 - 57 (Buryatiya),
• C 2n 39 - 41, NF 55 - 60 (Far East)
• D 2n 4043, NF 54-58 (Zabaikalskii kray and E.
  Mongolia)
• V 2n 36 - 40, NF 52 - 56 (Transbaikalia and
  W.Mongolia).

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5 chromosomal forms of M. maximowiczii
(Kartavtseva et al., 2008)
B 2n38-42
A 2n39-44
Russia
M. m. ungurensis
M. m. maximowiczii
Mongolia
D 2n40-43
C 2n39-41
V 2n40-43
China
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"A 2n39-44
M. m. ungurensis
M. m. maximowiczii
"C 2n39-41
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control region mtDNA
M. m. maximowiczii
M. m. ungurensis
?
?????????
?
D
?????
B
????????
V
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Material Map of chromosomal investigation

• We investigated karyotype of 36 individuals of
  Maximowicz's vole from six earlier not
  investigated populations of the territory Middle
  Amur river (chromosomal form C).
• Literature data
• 2n42
• 2n39
• Our data
• (2n40-41)
• New data

10
2n40-41
2n41
2n40
11
Chromosomal forms D and C
2n40
2n42
12
New chromosomal nomenclature of M. maximowiczii
by fluorescent in situ hybridization (FISH)
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M. maximowiczii (2350) from Romanovka
(chromosomal form A, our data)
11,20
11
2n41
M. maximowiczii FISH by Lemskaja et al., 2010
from chromosomal form A
13
M. Maximowiczii (2060)from Norskii reservation
(our data)
11
20
20
11
14
(No Transcript)
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Map of investigation of control region A Bmt DNA
940 np

• A

A
B
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Conclusion

• In the territory Middle Amur river (chromosomal
  form C)
• Stabilization of karyotype with Robertsonian
  rearrangement chromosomal pairs 11 and 20 and
  inversion of pair 14
• Two clasters of control region of mt DNA can be
  results of isolations of two animals groups.

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Thanks for attention