BC basic - from big bang - PowerPoint PPT Presentation
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BC basic - from big bang
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Title: BC basic - from big bang Author: Rong-Huay Juang Last modified by: juang Created Date: 8/15/1997 8:46:50 PM Document presentation format: – PowerPoint PPT presentation
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Title: BC basic - from big bang
1
How to Separate These Objects
Shape Size Density
wood stone cotton wood wood cotton stone wood
stone cotton stone cotton
Shape
4
6
Size
8
5
Density
7
Different rolling speed
Different sedimentation
Sieving different sizes
Juang RH (2004) BCbasics
2
Basic Principles of Protein Purification
Organelle
Homogenization
Small molecule
Cell Debris
Amino acid, Sugar, Nucleotides, etc
Protein
Nucleic acid
Carbohydrate
(Lipid)
Ammonium sulfate fractionation
Size
Charge
Polarity
Affinity
Ion exchange, Chromatofocusing, Disc-PAGE, Isoelec
tric focusing
Reverse phase chromatography, HIC, Salting-out
Gel filtration, SDS-PAGE, Ultrafiltration
Affinity chromatography, Hydroxyapatite
Juang RH (2004) BCbasics
