Gene%20Transfer

1
Gene Transfer

• Vertical
• DNA transfer to progeny
• Lateral (horizontal)
• Donation of DNA across the same generation

2
Bacterial Gene Transfer and Recombinant DNA
Technology

• Transformation
• Griffiths experiment

3
Transformation

• Mechanism
• Significance

4
Transduction

• Mechanism
• Significance

5
Conjugation

• Mechanism
• Significance

6
Plasmids

• F plasmid
• Resistance plasmids
• Virulence plasmids

• Tumor-inducing plasmids
• Plasmids conferring novel catabolism

7
Genetic Engineering

• Protoplast fusion
• Cell wall removal
• Genome fusions for plants, fungi, bacteria
• Nocardia

• Gene Amplification
• Recombinant DNA cloning

8
Polymerase Chain Reaction
9
Applications

• Medical
• Industrial
• Agricultural
• Hybridomas

10
Risks and Benefits

• ELSI
• Concerns