??????:Reference Manager 9.0

1
?????????
2
????????

• ??????????????,???????????????????????,???????????
  ?
• ??????Reference Manager 9.0

3
Reference Manager 9.0

• ?????????????PubMed?609?Z39.50?????????,??????????
  ?????
• ??????????????,?????????????????????????????????WO
  RD?????,??????
• ???????????????,????????????????????,?????????????
  ???,???????????

4
Reference Manager 9 ??
5
2. Endnote 3.1.2

• ?????????????,????????,???????????.

6
?? ??????

• ???????,??????????DNA?RNA?????????????,??
• ?????
• ????
• ??????
• ????
• ???(motif)??
• RNA??????
• ????????
• ?????????????

7
1? ????Omiga 2.0

• Omiga???????????????,????????????????????????????
  ??,???????
• ?Clustal. W????????,??????
• ??????????????(Motif)??????(ORF),?????PCR??????
• ??????????(Proteolytic Sites)??????????
• ??????????????,?????????????
• ??,?????????????????????????????,?????????????????
  ????

8
Omiga 2.0 ORF Map
9
DNASIS 2.5

• DNASIS for Windows 2.5????????(Hitachi Sofeware
  Engineering Co.,Ltd.)97??????????????????
• ??????????????????,???DNA?RNA????????????,????????
  ???????????,?????????????
• ?DOS??,DNASIS 7???????????????????????????,???????
  ??Win??DNASIS ????????

10
DNASIS 2.5 RNA ??????
11
DNATools 5.1

• ?Omiga, DNAsis, PCgene????????????,????????
• DNATools??????????,???????????????????????????????
  ?
• DNATools?????,??????????????????????????????(?????
  ????????),????????????,??????????????DNA??,???????
  ?????

12
DNATools

• ??????DNATools???(DNA???????),???????????,????????
  ??????????(????DNA?????????)?
• ??????????????????,?????????????????
• ?????????????????????????????????????????,????????
  ????

13
DNATools

• ????????????,DNATools???????????????5????/????,??
  ???????????,????????????????,?????????????????????
  ??

14
2?????????

• DNAssist 1.0
• ?????????????????,??cNNNNNNNNgaatt?????????EcoR
  I????
• DNAssist 1.0???????EcoR I??????
• ??DNAssist????????,??????????,????DNASIS?????,????
  ??(????,???????)?

15
3? ????

• Primer Premier5.0?????Premier?????????PCR????????
  ????????????,?Plasmid Premier2.02?????????????????
  
• ????????????(Genetank),??????(Primer
  Design),??????(Restriction Sites)?Motif?????

16
Primer Premier5.0

• ????,???????????????
• ?????????????????????????,?????????,??????????????
  ??????????????
• ???????,?????????,?????????????
• ???????

17
4? ???????(Alignment)

• GeneDoc 3.2 GeneDoc????????????????????,??????
  ???,??????????????????,???????????????,??????,????
  ?????

18
MACAW 2.05

• ?????????????(Multiple Alignment Construction
  Analysis Workbench, MACAW)??????????????????????

19
MACAW

• ??????
• 1. ???????????,?????????????
• 2. ???????????????block???????????
• 3. ????????,????????block??????????????
• 4. ???????????block???????????????????????,???????
  ?????????MACAW????????

20
Clustal X

• ?????????????????(multiple sequence
  alignment)????
• ???????????????????,
• ????????,
• ??????,
• ?????????,
• ????????????????,
• ??PCR??,
• ??? ????????????,
• Clustal X???????????

21
5? ????

• Gene Construction Kit 2.0????????????????????????
  ??,??????????????????
• ??????,???????????????(??????)???????????????????
  ?????????????????,????????????????????,?????,????
  ???

22
Plasmid Premier2.02

• ??????Premier Biosoft????????????????,??????????,?
  ???????????
• ?????????????(Genetank),??????(Plasmid
  Design),??????(Restriction Sites)???????(Motif)?
• ??????????????,??????Genbank?Vector?????????????,?
  ????,????????????,?????,???,?????????????????Heade
  r????????????????????

23
6? ???(motif)??

• Primer Premier 5.0??????????????????,????????????
  ???????
• ???????????????????????????????????????

24
7? RNA??????

• RNAdraw?????RNA??????????
• 1. ? ?Windows??????? (multiple document
  interface) ??,???????????????????????????????????
  ??????????????????????????????????
• 2. RNAdraw?????????????????????????????????????/??
  ??????????
• 3. RNA??(RNA Library)?????????????????RNA?????

25
RNAStructure 3.5

• RNA Structure ?????????,?Zuker???RNA??????RNA???
  ???????????
• ??????????????Turner????????????????Zuker?????,???
  ???????RNA?????

26
RNAStructure 3.5 RNA ?????
27
8? ????????

• ????Antheprot 4.5 ?????????ANTHEPROT
  4.5?????????????????(Institute of Biology and
  Chemistry of Proteins)???????????????????
• ??????????????????????,?????????????,??PC?,???????
  ????????????????????????????????????,?????????????
  ??????

28
DNASIS 2.5 ????????
29
9? ??????

• ????????????????????,???????????????,????????????
  ?????????????????
• ????RasMol 2.7.1

30
RasMol 2.7

• ???????????????????????????,????????????,?????????
  ,????Internet??????????,?????????????????
• ??RasMol 2.7??????????,????????????,??????????????
  ???
• ???????????????????????????????

31
RasMol 2.7 ??1EQF A?????
32
RasMol 2.7

• RasMol 2.7????GlaxoWellcome??(?????????)????????R
  oger Sayle?
• RasMol 2.7??????????,??????,??????,????????,?????
  ??????,?????DNA?RNA ????,?????????????????,???????
  ???????????????????????????????????????
• ??????,??????????????????????????

33
Cn3D

• ??NCBI?????????????????,??????????NCBI????????????
  ??MMDB???????????,
• ?????????????,?????,???????,?????????

34
(No Transcript)
35
Cn3D

• ????????,?????????????????,????????Rasmol?Weblabvi
  ew?????
• ???????,??????NCBI????????MMDB?????,??????????????
  ????????Entrez???????????????,?????????

36
Cn3D

• ?Cn3D??????????????????????????????,
• Cn3D???????????????Blast????????Genbank???????????
  ??????,????????????????????????

37
10? ????

• ???????????????????,????????????????????????????
  ??
• ????Seqverter 1.3

38
Seqverter 1.3

• ????,??????????????????????
• ????????????????????????
• ??,????????????????

39
11? ??????

• ????band leader 3.0 ????DNA????????????????
  ?????????????
• ???????????????,???????WINDOWS????
  .BMP,??????????

40
?? ????????

• 1?   ?????????Pov-Ray for Windows 3.1
  ???????,?????pov????,????????????????????????,???
  ??????????????????????????????????????12801024?
  
• ????molmol 2.5.1?????pdb????????????,??????????

41
????????? Sequin 2.90

• Sequin 2.90??GenBank?EMBL?DDBJ????????????
• ???????????????????,?????????,????????????????????
  ???,???????????,?e-mail?????

42
Digest

• DIGEST ????DNA?????????????.
• ??????????????,??????????????WISCONSI.920?,???????
  ????????????.
• ???Don Gilbert?????UREADSEQ,???????????????

43
PCMolecule

• PCMolecule ????????????,?????.mcm??????????

44
Pcrare

• PC-Rare??????????????????(OFD)???PCR???,???????
  ??

45
Redsoft Plasmid

• Redasoft Plasmid ??????????????????????????????
  ???
• ????????????????????,???????????????????????????
• ???web???????????????,????????????????????????????
  ??????????1000???REBASE????????

46
Redasoft Plasmid

• ?????????????????????????
• ????? (What You See Is What You
  Get)???????????????????????????
• ???????????????????
• ???????????????????????????????????????Windows????
  ?
• ???e-mail?????????

47
TreeView

• Tree View???????????????
• ?????NEXUS?PHYLIP??????????,?????,????????

48
?????

• ??????????????
• http//www.cbi.pku.edu.cn/chinese/documents/softdo
  c/

49
???

• The end!