Complete video

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Class Topics
Title Biology 4/23/07
Objectives

• To learn about DNA fingerprinting.

• Complete video Harvest of Fear
• GM drugs
• Cloning
• DNA fingerprinting
• "Far better it is to dare mighty things, to win
  glorious triumphs even though checkered by
  failure, than to rank with those poor spirits who
  neither enjoy nor suffer much because they live
  in the gray twilight that knows neither victory
  nor defeat."
• Theodore Roosevelt

Tuesday, December 08, 2015 348 AM
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Class Assignments
By When
What

• Chapter 11 Quiz/Essay 4/27/07
• Due this class period
• Due next class period
• Due in the future

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Grade Sheet
2A p. 157 (5 pts.)
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Essay possibilities

• Introduction
• What are you going to cover?
• Limit pronouns
• 4 body paragraphs
• 1. What is a GMO?
• 2. How are GMOs made?
• 3 4 opinion statements (on next slide)
• Conclusion
• What did you cover?

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Body paragraphs 3 and 4

• Opinion statements on
• What do you think the government should do about
  potential resistant weeds or insects due to
  excessive use of Bt or Round up Ready crops?
• Do you think GM foods should be labeled?
• Do you think GM crops should be used in
  developing countries?
• Do you think companies, like Monsanto, should own
  (holds patents) genes and sell them
• 3rd paragraph should be supporting your opinion
  with facts
• 4th paragraph should be supporting opinion
  against arguments

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Drugs

• GM drugs
• Clotting factor proteins for hemophilia
• Erythropoetin for anemics
• Human growth hormone
• Insulin - diabetics

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From http//esg-www.mit.edu8001/esgbio/rdna/rdn
a.htmloverview
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Cloning

• Dolly the sheep in 1996
• Egg cell extracted from sheep and enucleated
• Mammary cell extracted from Dolly
• The two cells were shocked to stimulate fusion of
  the cells
• Grown in culture
• Implanted in uterus of sheep
• Cloned sheep was born
• Cloned animals

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(No Transcript)
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From http//www.sciencecases.org/dog_cloning/dog_
cloning_notes.asp
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Quiz Review

• Cloning
• How to
• Drugs
• DNA fingerprint
• Technique
• Southern Blot
• Uses
• Recovering the Romanovs

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Ethical Issues

• Good or Baad?
• Problems

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DNA Fingerprinting

• RFLPs
• Restriction fragment length polymorphisms
• When restriction enzymes are used to cut DNA they
  leave it in different size lengths
• The pattern left by the RFLPs after a Southern
  Blot technique is a DNA Fingerprint
• Used commonly in criminal cases and forensics

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Southern Blot technique

• DNA of interest is cut by restriction enzymes
• Fragments are separated by gel electrophoresis
• Separates DNA by size
• Smaller fragments move farther than large
  fragments
• DNA is negatively charged, the ends of the gel
  are positively charged
• DNA bands are transferred (blotted) onto filter
  paper
• Radioactive tracers for visible bands

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DNA Fingerprinting

• A Confounding Confusing Confectionery Crime
• Recovering the Romanovs