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Complete video

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Title: Biology 4/23/07 Objectives: To learn about DNA fingerprinting. Class Topics Complete video Harvest of Fear GM drugs Cloning DNA fingerprinting – PowerPoint PPT presentation

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Title: Complete video


1
Class Topics
Title Biology 4/23/07
Objectives
  • To learn about DNA fingerprinting.
  • Complete video Harvest of Fear
  • GM drugs
  • Cloning
  • DNA fingerprinting
  • "Far better it is to dare mighty things, to win
    glorious triumphs even though checkered by
    failure, than to rank with those poor spirits who
    neither enjoy nor suffer much because they live
    in the gray twilight that knows neither victory
    nor defeat."
  • Theodore Roosevelt

Tuesday, December 08, 2015 348 AM
2
Class Assignments
By When
What
  • Chapter 11 Quiz/Essay 4/27/07
  • Due this class period
  • Due next class period
  • Due in the future

3
Grade Sheet
2A p. 157 (5 pts.)
4
Essay possibilities
  • Introduction
  • What are you going to cover?
  • Limit pronouns
  • 4 body paragraphs
  • 1. What is a GMO?
  • 2. How are GMOs made?
  • 3 4 opinion statements (on next slide)
  • Conclusion
  • What did you cover?

5
Body paragraphs 3 and 4
  • Opinion statements on
  • What do you think the government should do about
    potential resistant weeds or insects due to
    excessive use of Bt or Round up Ready crops?
  • Do you think GM foods should be labeled?
  • Do you think GM crops should be used in
    developing countries?
  • Do you think companies, like Monsanto, should own
    (holds patents) genes and sell them
  • 3rd paragraph should be supporting your opinion
    with facts
  • 4th paragraph should be supporting opinion
    against arguments

6
Drugs
  • GM drugs
  • Clotting factor proteins for hemophilia
  • Erythropoetin for anemics
  • Human growth hormone
  • Insulin - diabetics

7
From http//esg-www.mit.edu8001/esgbio/rdna/rdn
a.htmloverview
8
Cloning
  • Dolly the sheep in 1996
  • Egg cell extracted from sheep and enucleated
  • Mammary cell extracted from Dolly
  • The two cells were shocked to stimulate fusion of
    the cells
  • Grown in culture
  • Implanted in uterus of sheep
  • Cloned sheep was born
  • Cloned animals

9
(No Transcript)
10
From http//www.sciencecases.org/dog_cloning/dog_
cloning_notes.asp
11
Quiz Review
  • Cloning
  • How to
  • Drugs
  • DNA fingerprint
  • Technique
  • Southern Blot
  • Uses
  • Recovering the Romanovs

12
Ethical Issues
  • Good or Baad?
  • Problems

13
DNA Fingerprinting
  • RFLPs
  • Restriction fragment length polymorphisms
  • When restriction enzymes are used to cut DNA they
    leave it in different size lengths
  • The pattern left by the RFLPs after a Southern
    Blot technique is a DNA Fingerprint
  • Used commonly in criminal cases and forensics

14
Southern Blot technique
  • DNA of interest is cut by restriction enzymes
  • Fragments are separated by gel electrophoresis
  • Separates DNA by size
  • Smaller fragments move farther than large
    fragments
  • DNA is negatively charged, the ends of the gel
    are positively charged
  • DNA bands are transferred (blotted) onto filter
    paper
  • Radioactive tracers for visible bands

15
DNA Fingerprinting
  • A Confounding Confusing Confectionery Crime
  • Recovering the Romanovs
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