Things to Do Today

1
Things to Do Today

• Observe Unknown gm (-) results
• Start Urinalysis
• Start gm () ID
• Check for hemolysis
• Catalase test
• Coagulase test
• Direct stain colonies
• Observe Throat cultures
• Check for hemolysis
• Catalase test
• Gm stain isolated colonies

2
Oxidase Test
Positive
Negative
3
Urease Test
Negative Positive
4
Methyl Red Test
Negative Positive
5
KIA Slants
uninoculated
6
KIA Slants
uninoculated
7
Citrate test
Negative
positive
8
The Enterotube Multitest System
9
The API System Various species of
Enteobacteriaceae are shown here with differences
in reactions that enable them to be identified
10
Things to Do Today

• Observe Unknown gm (-) results
• Perform oxidase test with culture from KIA slant
• Observe Urease tubes
• Perform MR test by adding 6-8 drops MR reagent to
  the culture
• Observe KIA slants for the various reactions

11
Three species of Candida can be differentiated in
mixed culture when grown on CHROMagar Candida
plates
12
Identification of urinary tract pathogens with
differential media (CHROMagar)
13
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14
EMB with E. coli
Used with permission
15
EMB
Enterobacter aerogenes
Pseudomonas auginosa
16
Fig. 15-22
Relative Frequencies of Nosocomial Infections I
John Wiley
17
Fig. 15-19
Relative Frequencies of Nosocomial Infections II
John Wiley
18
Things to Do Today

• Start Urinalysis
• Do a 4 quadrant streak with the urine sample
  using a metal loop onto the EMB plate
• Perform the streak with the urine sample
  using the plastic loop onto the BAP plate

19
A tale of two Staphs
20
BAP Hemolytic Reactions
Used with permission
21
Throat Swab
Used with permission
22
Catalase test
Positive
negative
23
Coagulase test

• Place a drop of the red latix suspension on the
  card.
• Mix a tiny portion of the colony into the mix.

24
Things to Do Today

• Start gm () ID
• Direct stain with Crystal Violet
• Check for hemolysis
• Catalase test
• Coagulase test
• Observe Throat cultures (Do in this order)
• Check for hemolysis
• Catalase test and find cat (-) colony
• Gm stain isolated colonies on slide with throat
  swab

25
(No Transcript)
26
BAP Hemolytic Reactions
Alpha hemolysis
Beta hemolysis
gamma
27
Types of hemolysis A Alpha or partial
hemolysis of red blood cells results in a
greenish zone B B-hemolysins, which produce
complete breakdown of hemoglobin, causing clear
zones to form around colonies on blood agar
B
A