BIOE/CHBE 460

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BIOE/CHBE 460 Biochemical Engineering
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Example of process improvement
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Major advancements

• Molecular biology
• Genetic engineering
• Cell and tissue culture

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Examples of genetic engineering
application (first generation application)
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Recombinant proteins by microorganisms

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Recombinant proteins by microorganisms
Some early products
Year Products Disease Company 1982 Humulin
Type 1 diabetes Genetech, Inc. (synthetic
insulin) 1985 Protropin Growth hormone
Genetech, Inc. Deficiency
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Examples of pathway and metabolic
engineering (second generation application of
genetic engineering)
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Introduction
What is pathway and metabolic engineering?
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chilling tolerant plant
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Cell and tissue cultures monoclonal antibody by
hybridoma cells
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antigen injected
into mouse
spleen removed
myeloma cells
B lymphocytes
hybridoma
cells grown in HAT medium
only hybridoma cells grow
screen for desired clone
Monoclonal Antibody
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Applications of Monoclonal Antibodies

• Diagnostic
• ELISA Tests
• PSA, AIDS, pregnancy
• Passive Immunization
• Detection, isolation and purification of
  biomolecules

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A Fermenter / Bioreactor And Its Parts
by Genentech, Corporate Communication
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Separation
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Chromatography Columns
BioProcess Glass (BPG) Columns
Amersham Pharmacia Biotech
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