BIOE/CHBE 460 - PowerPoint PPT Presentation

About This Presentation
Title:

BIOE/CHBE 460

Description:

... by hybridoma cells Monoclonal Antibody screen for desired clone only hybridoma cells grow cells grown in HAT medium hybridoma myeloma cells ... technology ... – PowerPoint PPT presentation

Number of Views:100
Avg rating:3.0/5.0
Slides: 24
Provided by: ksa72
Category:

less

Transcript and Presenter's Notes

Title: BIOE/CHBE 460


1
BIOE/CHBE 460 Biochemical Engineering
2
(No Transcript)
3
Example of process improvement
4
Major advancements
  • Molecular biology
  • Genetic engineering
  • Cell and tissue culture

5
(No Transcript)
6
(No Transcript)
7
(No Transcript)
8
Examples of genetic engineering
application (first generation application)
9
Recombinant proteins by microorganisms

10
Recombinant proteins by microorganisms
Some early products
Year Products Disease Company 1982 Humulin
Type 1 diabetes Genetech, Inc. (synthetic
insulin) 1985 Protropin Growth hormone
Genetech, Inc. Deficiency
11
Examples of pathway and metabolic
engineering (second generation application of
genetic engineering)
12
Introduction
What is pathway and metabolic engineering?
13
(No Transcript)
14
(No Transcript)
15
chilling tolerant plant
16
Cell and tissue cultures monoclonal antibody by
hybridoma cells
17
antigen injected
into mouse
spleen removed
myeloma cells
B lymphocytes
hybridoma
cells grown in HAT medium
only hybridoma cells grow
screen for desired clone
Monoclonal Antibody
18
Applications of Monoclonal Antibodies
  • Diagnostic
  • ELISA Tests
  • PSA, AIDS, pregnancy
  • Passive Immunization
  • Detection, isolation and purification of
    biomolecules

19
(No Transcript)
20
A Fermenter / Bioreactor And Its Parts
by Genentech, Corporate Communication
21
Separation
22
Chromatography Columns
BioProcess Glass (BPG) Columns
Amersham Pharmacia Biotech
23
(No Transcript)
Write a Comment
User Comments (0)
About PowerShow.com