Report your results

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Report your results
Group LB only LB-amp LB-Kan LB-Amp/Kan
(-) control Lawn NG NG NG
() control pAMP Lawn 100 colonies (satellites) NG NG
() control pKAN Lawn NG 2 gigantic symmetrical contaminant mold colonies NG
Biniam and Joanna Lawn NG NG NG
Brian, John Tenley Lawn 1 colony (w/satellites) NG NG
Hua and Qian Lawn NG NG NG
Alex, Zubeda Lawn NG NG NG
Samson, Greg, Shiwei Lawn NG NG NG
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iGEM

• Principle of parsimony
• occams razor

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What is iGEM?

• iGEM stands for iNternational Genetically
  Engineered Machines
• An undergraduate bioengineering competition
• Heavily influenced by the subdiscipline Synthetic
  Biology

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MIT is the host of iGEM

• iGEM Jamboree held in November
• Work done in the summer
• iGEM 2009 will be the 7th annual

5
iGEM is truly international

•   Alberta NINT
• Bay Area RSI
• BCCS-Bristol Best Model
• Beijing Normal
• BolognaBest Experimental Measurement
• Brown Best Environment Project
• BrownTwo
• Calgary Ethics
• Calgary Software
• Calgary Wetware
• Caltech Finalist 2nd Runner Up, Winner of the
  Synthetic Standard
• Cambridge
• Chiba
• Colombia
• CPU-NanJing
• Davidson-Missouri Western
• Duke
• Edinburgh

• Slovenia Grand Prize, Winner of the BioBrick
  Trophy
• Tianjin
• Tokyo Tech
• Toronto Bluegenes Withdrew
• Tsinghua
• TUDelft Best Wiki
• TU Munchen Withdrew
• UCSF Best New Application Area Best Poster,
  Runner Up"
• UC Berkeley
• Finalist
• UC Berkeley Tools
• Best Software Tool
• UNIPV-Pavia
• University of Alberta
• University of Chicago
• University of Lethbridge
• University of Ottawa
• University of Sheffield

6
iGEM is related to the subdiscipline of synthetic
biology

• Synthetic Biology- the incorporation of DNA
  manipulation and biotechnology to engineering
  applications
• Other important foci
• Standardization
• Open Source

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Standardization has deep roots in engineering

• With standard threads, nuts bolts become
  interchangeable
• Builders have significantly fewer headaches
• Other standards Wiki,

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BioBricks are standardized pieces of DNA

• Standard pieces have standard restriction sites
• Allow easy assembly and recombination
• They can (and must!) be shared
• Innovation a new way of doing something

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Student teams share the DNA parts they create
with the Registry of Standard Biological Parts

• 3200 genetic parts that can be mixed and matched
  
• Founded in 2003 at MIT,
• part of the Synthetic Biology community's efforts
  to make biology easier to engineer.
• provides a resource of available genetic parts to
  iGEM teams and academic labs.

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Enzymes in biochemical pathways can be reorganized

• Pathways can resemble electrical circuits
• Circuits can be designed
• They can be modeled mathematically

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Open access to standard DNA sequences accelerates
innovation

• And cheapens it!
• Registry of Standard Biological Parts now has
  thousands of sequences
• Sequences can be sent in the mail, and used by
  anyone
• Pieces can be used to create novel GMOs

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There has never been a community college iGEM team

•   Alberta NINT
• Bay Area RSI
• BCCS-Bristol Best Model
• Beijing Normal
• BolognaBest Experimental Measurement
• Brown Best Environment Project
• BrownTwo
• Calgary Ethics
• Calgary Software
• Calgary Wetware
• Caltech Finalist 2nd Runner Up, Winner of the
  Synthetic Standard
• Cambridge
• Chiba
• Colombia
• CPU-NanJing
• Davidson-Missouri Western
• Duke
• Edinburgh

• Slovenia Grand Prize, Winner of the BioBrick
  Trophy
• Tianjin
• Tokyo Tech
• Toronto Bluegenes Withdrew
• Tsinghua
• TUDelft Best Wiki
• TU Munchen Withdrew
• UCSF Best New Application Area Best Poster,
  Runner Up"
• UC Berkeley
• Finalist
• UC Berkeley Tools
• Best Software Tool
• UNIPV-Pavia
• University of Alberta
• University of Chicago
• University of Lethbridge
• University of Ottawa
• University of Sheffield

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Possible iGEM proposals

• Using Stem Cells
• Using Anaerobic iron-reducing bacteria to create
  a battery
• Using Electric Eel Cells to create a battery

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What we need

• Standard team budgets run 45k
• We could do with less

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iGEMs standard budget- yikes!
expense cost / unit units total
summer stipends 4,000 / student 7 students 28,000
iGEM Team Fee 1250 / team 1 team 1,250
Jamboree Fee 175 / undergraduate student 7 students 1,250
Jamboree Fee 375 / advisor / graduate student / other 3 advisors 1,125
flights to Jamboree 500/person (roundtrip) 10 people 5,000
Hotels near Jamboree 250 / night 2 people / room 5 rooms x 3 nights 3,750
40,350
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• How we can get more money
• -Private companies
• -Bake sales
• Limits on team size
• Demands on team time
• What we can do if we get less money than we want
• What we can do if we get no money at all

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Possible team members

• Leeza Sergeeva
• Greg Kielian
• Dan Ingwersen
• Renhao Zhang
• Colby Sandate
• Angela Brock
• Bertram Lee
• Songyao Cui