UV Sensor

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UV Sensor

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?????UV???????
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UV Sensor
UV
RecA
Protease

c I
Lex A
Lex A
c I
UV??? LexA-LexA???(SOS?????)
cI-cI???(????????)
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?SOS?????
DNA????????
recA
umuC
???????
LexA
uvrA
uvrB
sfiA
???????
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BBa_J22136
Detection of DNA Damage by Use of Escherichia
coli Carrying recA9lux, uvrA9lux, or
alkA9lux Reporter Plasmids AMY C. VOLLMER et
al(1997)
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?UV???cI repressor ??
UV dose
20J/m2
10J/m2
7.5J/m2
5J/m2
1J/m2
Host responses influence on the induction Of
lambda prophage Assaf Rokney et al(2008)
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UV???????????????
UV Source Mineralight lamp uvgl-58 16W
254nm
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UV?????????????
20µl LB180µl
1/10
60
50
40
30
20
10
0
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Bioassay medium
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??????
Type1
ltagt Double Transformation
C.N.1012
C.N.100300
ltßgt On low copy plasmid
BBa_S01416(??????) colony
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?SOS?????
SOS Operons
DNA Polymerase ?
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Part No. Detail Length(bp) Plasmid Plasmid Colony ?????? Insert Check ?? ????
BBa_J06650 PcI-EYFP-T 935 pSB1A2 pSB1A2 OK OK OK ?
BBa_R0051 PcI 49 49 pSB1A2 OK OK NG -
BBa_J22136 RecA-mRFP 898 898 pSB1A2 OK OK OK ?
BBa_J22141 RecA-mRFP-T 1035 1035 pSB2K3 ? NG NG
BBa-S01416 PtetR-cI?-T-PcI 1049 1049 pSB2K3 NG