Fundamentals of Microbiology Biol 315

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Fundamentals of MicrobiologyBiol 315
Lab 2 Instruments and common chemicals in
microbiology, Microscopy, Sterilization techniques
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Common Instruments

• Autoclave (121?C, 15 psi)
• Oven (0-300 ?C)
• Incubator (0-120 ?C)
• pH Meter
• Hotplate and Magnetic Stirrer
• Laminar Flow Cabinet / Biosafety Cabinet

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AUTOCLAVE
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http//textbookofbacteriology.net/autoclave1.jpg
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http//i01.i.aliimg.com/photo/v0/108072579/Autocla
ve_Portable_Autoclave_Laboratory_Autoclave_Laborat
ory_Equipments.jpg
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http//www.naanolabindia.com/laboratory-instrument
s/vertical-autoclave.jpg
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http//www.pharmaceutical-int.com/upload/image_fil
es/articles/images/companies/865/large-capacity-au
toclave-b.jpg
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http//www.theprominex.com/gallery/images/medical/
autoclave.jpg
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Oven
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http//2.imimg.com/data2/FU/BT/MY-2143786/1-250x25
0.jpg
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http//images.hellotrade.com/data/T/W/MY-633236/Ho
t-Air-Oven---Lab-Model_250x250.jpg
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http//faculty.ksu.edu.sa/baddour/PublishingImages
/hotairoven.jpg
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INCUBATORS
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http//www.stuart-equipment.com/adminimages/Incuba
tors_SI505_open.jpg
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http//www.stuart-equipment.com/adminimages/Incuba
tors_SI505_closed.jpg
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Shaking Incubator
http//www.ika.com/ika/product_art/images/3510000_
1.jpg
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pH Meter
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http//growsetup.files.wordpress.com/2011/02/3505-
ph-meter.jpg
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Hotplate and magnetic stirrer
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http//www.labdepotinc.com/admin/uploads/scilogex-
ms-h-s-m-10008-vlarge-r_1.jpg
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http//www.troemner.com/images/hotplate_stirrers/a
dv_hps_lge.jpg
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http//pimg.tradeindia.com/00076902/b/0/Laboratori
es-Magnetic-Stirrer-Hotplate.jpg
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Biosafety cabinet
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http//www.terrauniversal.com/gallery/hoods/Images
/biological_safety_cabinet_purifier_Cover.jpg
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Class I Cabinets

• These cabinets have unrecirculated airflow away
  from the operator that is discharged to the
  atmosphere after filtration through a HEPA
  filter.
• They provide good operator protection but do not
  protect the material within the cabinet (the
  product) from contamination.

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Class II Cabinets

• Class II cabinets are designed for personnel,
  product and environmental protection.
• They are designed for work involving
  microorganisms in containment levels 2, 3 and 4
  laboratories.
• Class II cabinets are most commonly used in
  biomedical research laboratories because of their
  characteristics.

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http//escoglobal.com/products/images/products-big
/1322810342.jpg
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Class III Cabinets

• Class III cabinets are totally enclosed and
  gas-tight with HEPA filtered supply and exhaust
  air. Work is performed with attached long-sleeved
  gloves.
• The cabinet is kept under negative pressure and
  airflow is maintained by a dedicated exterior
  exhaust system.
• Class III cabinets protect the worker and the
  product. They are designed for work with level 4
  pathogens.
• The exhaust air is double HEPA filtered or
  treated by HEPA filter and incineration.

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http//www.phac-aspc.gc.ca/publicat/lbg-ldmbl-04/g
fx/fig6_e.gif
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http//doh.sd.gov/img/bsc.jpg
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http//www.nap.edu/books/0309075718/xhtml/images/p
20005395g49001.jpg
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http//escoglobal.com/products/images/products-big
/AC2-D.jpg
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http//web.princeton.edu/sites/ehs/biosafety/biosa
fetypage/BSC2.jpg
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Chemicals

• Media (solid and liquid)
• Nutrient agar, nutrient broth
• EMB agar, MacConkeys agar, Blood agar
• Lactose broth
• Grams staining kit
• Catalase reagent
• Oxidase reagent

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Microscopy

• Light microscope
• Photography and video making through microscope
  using live camera
• Other kinds of microscopy

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Grams Staining result
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http//janiceatmeredith.files.wordpress.com/2012/0
1/gramsta.gif
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THANK YOU