Things to Do Today

1
Things to Do Today

• Observe Urinalysis Plates
• Note hemolysis Count Colonies on BAP
• Observe EMB plate
• Blood Lecture
• Blood Typing
• Differential WBC Count
• Observe Parasitic Blood Slides

2
Whole Blood

• Plasma
• Buffy coat
• Hematocrit

3
RBCs
4
RBC Disorders

• Anemias
• -hemolytic
• -Iron deficiency
• -aplastic
• -sickle cell
• Polycythemia

5
WBCs
Wikipedia Commons, used with permission
6
WBCs
neutrophil
eosinophil
basophil
monocyte
lymphocyte
Wiley
7
WBCs
WebPath, used with permission
8
WBCs
Vs.
lymphocyte
monocyte
Medicine USC, used with permission
9
Leukocyte Disorders

• Leukemia
• Infectious mononucleosis
• Leukopenia

10
Formation of platelets
11
Clotting
12
Blood Types
13
Hemolytic DZ of the Newborn

• Mom must be Rh (-)
• Father must be Rh ()
• A child must be Rh ()
• A subsequent child must be Rh ()

14
Hemolytic DZ of the Newborn
15
Blood typing
16
Watch demo of Blood Smear
10 sec in dye 20-24 sec in rinse
17
Blood Parasites
Microbe
Disease

• Trypanosoma cruzi Chagas
  disease
• Leishmania Leishmaniasis
• Plasmodium vivax Malaria
• Trypanosoma gambiense Sleeping sickness

18
Trypanosoma cruzi
1000x
Etiological Agent for Chagas disease
KM
19
Rhodnius Vector for Chagas disease
AKA assassin bug kissing bug
KM
20
(No Transcript)
21
Blood Parasites
Microbe
Disease

• Trypanosoma cruzi Chagas
  disease
• Leishmania Leishmaniasis
• Plasmodium vivax Malaria
• Trypanosoma gambiense Sleeping sickness

22
Leishmania Life Cycle
23
Leishmania
Vector sandfly
1000x
KM
24
A Patient Suffering from leishmaniasis
25
leishmaniasis
DermAtlas, used with permission
26
leishmaniasis
DermAtlas, used with permission
27
leishmaniasis
DermAtlas, used with permission
28
leishmaniasis
Dermis.net, used with permission
29
leishmaniasis
Dermatology Image Atlas, used with permission
30
(No Transcript)
31
Blood Parasites
Microbe
Disease

• Trypanosoma cruzi Chagas
  disease
• Leishmania Leishmaniasis
• Plasmodium vivax Malaria
• Trypanosoma gambiense Sleeping sickness

32
Malaria

• Causative agent Protozoan Plasmodium
• One of the most severe of all parasitic diseases
• Endemic is most tropical areas
• Plasmodium are amoeboid, intracellular protozoa
  that infect erythrocytes and other tissues
• Transmitted by the female Anopheles mosquito

33
The Worldwide Incidence of Malaria
34
The life cycle of the malaria parasite Plasmodium
35
Malaria in RBCs

The ring stage of the malarial parasite
Plasmodium falciparum Seen as dark, circular
structures within red blood cells
36
Plasmodium vivax
Malaria
1000x
KM
37
(No Transcript)
38
Blood Parasites
Microbe
Disease

• Trypanosoma cruzi Chagas
  disease
• Leishmania Leishmaniasis
• Plasmodium vivax Malaria
• Trypanosoma gambiense Sleeping sickness

39
Trypanosoma gambiense
1000x
Sleeping Sickness
Vector Tsetse fly
KM
40
(No Transcript)
41
(No Transcript)
42
Blood Types
Type A
Type B
Type O
Type AB
Anti A antibody
Anti B antibody