Hands-On

1
Hands-On
2
?????--?????In-Vitro Electroporation using
Cuvettes
3

• Check List
• Safety stand, electroporation buffer (with DNA),
  cuvettes, pipettes.
• ??
• 1??????
• 2??DNA????
• 3????
• 4????

4

• Remove the cuvette from package
• ??????????

5

• Pipette sample from 50 ml tube
• ????50ml

6

• Load sample into cuvette
• ????????

7

• Make sure the sample is settled between the
• 2 aluminum plates. Rid of bubbles in the cuvette.
• ???????????,?????????

8

• Place cuvette into safety stand, making sure
  metal contacts of safety stand touches the
  cuvette. Use the black roller to adjust the gap
  between metal contacts.
• ???????????(????????????),???

9

• Close lid of safety stand before pulsing
• ?????

10

• Make sure all cables are connected well. Push
  start on the instrument to deliver pulse.
• ??????????,????,????

11

• After pulsing, sample will foam depending on the
  buffer type and electrical parameters.
• ???,?????(???????????????)

12

• Plate the sample into plates for further assay.
• ???????,????????

13
Thats AllFOLKS!