Supplemental figure 1

1
Supplemental figure 1
A
B
C
Root
Nodule
SNP (mM)
kDa
kDa
Wt Fix-
kDa
- NO3-
NO3-
- NO3-
NO3-
0 1 10
150
150
150
100
100
100
75
75
75
50
50
50
37
37
37
25
25
25
Supplemental Figure1 Western blots of total
soluble proteins of M.truncatula root nodules
(10 mg per lane). The position of the molecular
weight markers is indicated. A- Total soluble
proteins were extracted from root nodules formed
by wild type (WT) and fixJ (Fix-) mutant strains
of S. meliloti. B- Total soluble proteins were
extracted from roots and from 21 day old root
nodules collected from plants treated for 2 days
with NH4NO3. C-Total soluble proteins were
extracted from root nodules collected from plants
treated with increasing concentrations of SNP.