The miracle of N2ase:

1
The miracle of N2ase How does it do it?
N
N
N2 bond energy 945 kJ/mol!!!!
N2 8H 8 e- 16 MgATP ? 2 NH3 H2 16
MgADP 16 Pi
2
Root nodules formed by Rhizobium
bacteria living symbiotically through nitrogen
fixation.
3
N2 8H 8 e- 16 MgATP ? 2 NH3 H2 16
MgADP 16 Pi
4
EPR used to monitor S 3/2 signal under a variety
of conditions
FeMo protein (FeMoco site)
Fe protein
5
Big Mystery What is this atom???
6
P-cluster
Reduced state
Oxidized state
Strange Sulfur CN 6 Creates flexibility for
Fes?
7
(No Transcript)
8
(No Transcript)
9
http//www2.pt.tu-clausthal.de/atp/projects/nitro.
html
Nitrogen Binding to the FeMo cofactor of
Nitrogenase, Johannes Schimpl, Helena M.
Petrilli, and P.E. Blöchl, J. Am. Chem. Soc. 125,
15772-15778 (2003), TU Clausthal Institute of
Theoretical Physics Department of Applied
Theoretical Physics
10
(No Transcript)
11
(No Transcript)
12
(No Transcript)
13
(No Transcript)