Nucleic acids

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Nucleic acids

• Sarote Boonseng

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Introduction

• ????????????????????????????
• Deoxyribonucleic acid (DNA)
• Ribonucleic acid (RNA)
• ?????????????????????????

???????? ????????? ???????????? ???????????
????????????????? DNA ??????? cytoplasm
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???????????? (DNA RNA)
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?????????

• ??????????????????
• ??????
• ??????

monophosphate
diphosphate
triphosphate
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Nucleoside
Nucleoside sugar base
N-glycosyl linkage
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Nucleotide
Nucleotide phosphate sugar base
5
5
phosphoester
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????????????????????
Nucleotide Abbreviation
Adenosine 5-monophosphate Adenosine 5-diphosphate Adenosine 5-triphosphate Deoxyadenosine 5-monophosphate Guanosine 5-monophosphate Cytidine 5-monophosphate Uridine 5-monophosphate Deoxythymidine 5-monophosphate AMP ADP ATP dAMP GMP CMP UMP dTMP
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DNA RNA
Deoxyribonucleic acid (DNA) A G T and
C Ribonucleic acid (RNA) A G U and C
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Watson-Crick base-pairing
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DNA

• ?????????????????????? ??????? ???????????????????
  ???
• ????????????????????????? DNA ???? Antiparallel
• ?? DNA ?????????????? ?????????
• DNA ????????????????????? RNA
• DNA ?????????????????????? nucleosome (DNA ??????
  Histones) ?????????????? chromosome

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RNA

• ????????????????????????? ??????????????????????
  ????????????????????????? Hair-pin structure
• ?? RNA ?? cytoplasm ???
  Nucleus
• ???????? 3 ????
• mRNA ???????????????????????
• rRNA ?????????????????????????
• tRNA ???????????????????????????????
  

????????? ???????????
??????? RNA ?????????????????
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Properties of nucleic acid

• Denatured DNA
• Break down H-bonds by heat or alkaline
• ?????????????? DNA ?????????????????????

Supercoiled form Linear form Circular form
D Supercoiled gt Linear gt Circular
????? http//www.sbs.utexas.edu/395J/395J_2009/Re
adings/Lecture202/Supercoiling.pdf
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?????????????????????
H/heat
DNA/RNA
Base
Sugar
PO4
Deoxyribose
Ribose
Diphenylamine
Orcinol
Purple color
Green
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????????????????
DNA ?????????????????????? RNA ???????????????????
????? ????? C2 ??? RNA ???? OH ????
rearrangement ??? phosphate
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The End