??????????????????NAD ?FAD???????????????????,??????????????,??????????????,?????????????????????????????????????????????,??????(biological

1
???????????????????2????????????

• ??????021?
• ???

2
??

• ??????????????????NAD?FAD???????????????????,
  ??????????????,??????????????,????????????????????
  ?????????????????????????,??????(biological
  oxidation)????????????????????,????????????????,??
  ??????????????????,????????,????????????pH???????,
  ?????????????????????????,????????????????????????
  ????????????,??????????(?ATP?GTP?)?,?????????????

3
??

• ??????????????????????????????????????????????????
  ?????
• ??????????????????????,ADP??????ATP??????

4
????????

• ?????????????????????ATP????, ????????????????????
  ?,??????????????????????,?????????????????????????
  ????????????

5
????

• 1968?E.Racker????????????,???????????????????,
  ?????????????(submitochondrial vesicle)???????(sub
  mitochodrial particles)???????????????????????????
  ???????,?????????????,??????????????,????ADP?????A
  TP? ????????????????????,?????????????????????????
  ?,?NADH????????????????????????????????,??????????
  ????,????????,????(ATP????)??????

6
????????????

• ?????
• ATP???????????
• ???????????????
• ??????????????

7
??

• ??????????,???????????????????,?????????ATP????
  ?ATP??????????????????????????????????????????????
  ???????

8
THANK YOU!
9
?????

• ?????(respiratory chain)????????(electron
  transport chain),?????????????????????????????????
  ??????????????,??????????????????????????????????
  ??,???NAD?FMN?FAD?UQ?????????,??????????????????
  ???????????????????????????????NAD?FAD?UQ???????
  ?O2?

10
????????

• ???I
• ??Q
• ???II
• ???III
• ????c
• ???IV

11
??????

• 1.?????????????????? ????????????????????????????
  ???,??????,?????
• 2.??????????????????? ????H?????????????????????
  ??NADH???O2?,???6?H??FADH2??,????4?H?????H,????
  ?????????,??????????,??????????????(?pH)?????(?E),
  ???????H???????µH,???µH??????V???,???????
• ?????????,????????,????ATP??????

12

• 3.???????ATP??? ?????H???ATP?(????)?H??????????
  ,????????ADP?Pi??ATP(??)?
• ??????????????????????????O2????,??O2?,??????,???H
  ???????1.5pH??,????0.5V,?????????????,???????,???
  ???????H??????????????????????2,4-?????(DNP)?,???
  H????????????,????ATP???????????????????ATPase???
  ??,????ADP?Pi???,??????????????H,?????H???,??ATP
  ?????????????????H????,???ADP?Pi???ATP?

13
????????????????  
14
1.????

• ??NADH???????(NADHubiquinone oxidoreductase)?
  ???700X103900X103,??25???????,?????????(flav in
  mononucleotide,FMN)???????????????,?????????(iron
  sulfur protein,IP)???????(iron?sulfur
  flavoprotein,FP)???(ubiquinone,UQ)???(phospholipid
  )?????????????????????TCA?????NADHH??2?H?FMN???
  ????,?????Fe-S?2??????UQ(????Q,CoQ)UQ??????H??,?
  ??????(ubiquinol,UQH2)???????????(rotenone)??????A
  (piericidin A)?????(barbital acid)??????????????,?
  ???Fe-S???????????

15
(No Transcript)
16
2.????

• ?????,???????(succinateubiquinone
  oxidoreductase)????140103,??45???????,??????????
  ?(succinate dehydro genase,SDH)??????????(flavin
  adenine dinucleotide, FAD)?????b(cytochrome
  b)?3?Fe-S???????????????????????,??H???FAD??FADH2,
  ????H???UQ??UQH2???????2-????????(thenoyltrifluoro
  acetone,TTFA)????

17
(No Transcript)
18
3.????

• ??UQH2????C?????(ubiquinonecytochrome c
  oxidoreductase),???250103,??910??????,?????2?Cyt
  b,1?Fe-S???1?Cyt c1??????????????UQH2?Cyt
  b?FeS?Cytc1???Cyt c,??????????????,??2?H?????????
  ????????Fe-S??Cyt c1??,????UQ,??UQ?????H????UQH2?
  UQH2??????Cytc1,???2?H????????

19
??????????(????c ?????)???????????
20
4.????

• ??Cyt c???????(Cyt ccytochrome
  oxidase)????160170103,??????????,?????Cyta?Cyta3
  ?2????,???????????Cyta CuA?Cyta3
  CuB,??????????Cyt c ?????,?????????????????Cu
  Cu2???,?Cyta?Cyta3??????????? Cyt
  c??????????,????Cyta3?CuB??????????????????,O-O??
  ?,??????????H2O????????????,?????????????????????
  ???????????? 2?H????????CO????(cyanide,CN-)?????(
  azide,N3-)?O2???Cytaa3?Fe???,????Cytaa3?O2??????

21
???????(????c???)??????????? 
22
???????(??)

• ??????FADH2
• ???-Q??? ?
• NAD?NADH-Q?Q??????????c?
• ??? ???
• ?????????

23
? 5-11 ?????????????????? ?????????????????????
UQ??????????????
24
ATP???

• adenosine triphosphate synthase,??H-ATP??????8???
  ????,??????8.210355.2103,???????????????(F1-F0)
  ?F1????????,??????,?????,????????????F0??,????????
  ,??????,???????????????????????,?ADP?Pi??ATP,?????
  ??????????????????ATP???

25
(No Transcript)
26
? 5-14 ATP????????????? F1????????????????????????
?????????????????(L),?????????(T)??????????(O)????
???F1?????????????,?????????????????????ADP?Pi????
?????????????(1)???????F0?????????????????????????
?????????????ATP?T?????O?,ATP????????,???ADP?Pi?L?
????T?,??????????ATP?????????ADP?Pi?????????????(2
)???????ADP?Pi????ATP,??????????????(3)  
27
??????????????

• 1.????(uncoupler)
• 2.???(depressant)
• 3.???????(ionophore depressant)

28
1.????(uncoupler)

• ?????????????????,???ADP??ATP??????,????????,?????
  ??????????????,???????????????,???????????????????
  ????????????????????????????????DNP,?????????DNP??
  ???,?????????,????H???????,?????????????,???ATP??
  ????????????????,O2??????

29
2.???(depressant)

• ?????????????,????????ATP???,?????O2????????????(o
  ligomycin)??????????????????H??ATP????Fo???????,?
  ??????ATP??,????????????,???????????,O2??????????

30
3.???????(ionophore depressant)

• ????????????????????H??,????????????,?????????,??
  ????????????????,????????????????????????????????
  ???????,???????,?????ATP??????????(valinomycin)?K
  ????????????????,???????????

31
???????????????

• ?????????????????????????????,??H??????H ?e-
  ?????NADH?FADH2 ????????????,????ADP?????ATP,?????
  ???????????????ATP???????,??????????????????,?????
  3???????????,?3????????????????????ADP??????????,?
  ????????????????????NADH?????ATP
  ?3?????NADH???Q?????b?????c?????aa3
  ?????3???????ATP,???3?ATP????FADH2
  ??????2?ATP??,???????FADH2 ???Q???????????????????
  ??

32
??Q

• ??Q(CoQ)????,?????Q????????????????????????,??
  ?????,??????????????????????????????????????NADH-Q
  ????????????,????????????????????????CoQ??????????
  ????

33
????c

• ????c?????????13 000????????,???3.4nm,?104?????
  ????????????????????????????c?????????????????????
  ?????,???????????????????????
• ????c???????III?????C1 ????IV??,??????III?IV??
  ????????